| 米非司酮诱导大鼠PCOS动物模型的实验研究 |
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| 引用本文： | 杨智莺,肖国宏,侯棚钟,刘贞,杨洁,陈敦金. 米非司酮诱导大鼠PCOS动物模型的实验研究[J]. 现代妇产科进展, 2011, 20(8): 637-640 |
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| 作者姓名： | 杨智莺 肖国宏 侯棚钟 刘贞 杨洁 陈敦金 |
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| 作者单位： | 广州医学院第三附属医院妇产科,广州市妇产科研究所,广州 510150 |
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| 基金项目： | 广州市教育局科技攻关项目，广州市教育局团队项目 |
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| 摘 要： | 目的:建立抗孕激素米非司酮诱导成年雌性SD大鼠多囊卵巢综合征(P-COS)动物模型,并探讨其意义。方法:选择4～5天规律动情周期的成年雌性SD大鼠,于动情周期第一天皮下注射米非司酮并阴道涂片监测动情周期,8天后称重大鼠,取出卵巢,称重并观察大体解剖及光镜(HE)改变,测定血清LH、T、FSH、E2水平。结果:(1)用药后第4天动情周期开始失去规律性,阴道上皮呈持续角化,提示无排卵。(2)大鼠体重及卵巢单位重量明显增加(P<0.01)。(3)肉眼观察:大鼠卵巢表面的卵泡明显扩张,卵巢色泽及白膜厚度未见明显异常。镜下观察:大鼠卵巢白膜下见多个呈囊性扩张的卵泡及闭锁卵泡,颗粒细胞层减少至2～3层,卵泡内卵母细胞或放射冠消失,黄体数量明显下降并伴有不完全的黄素化。(4)血清LH、T、E2显著升高(P<0.01),FSH浓度降低,但差异无统计学意义(P=0.094)。结论:米非司酮诱导的成年雌性大鼠PCOS模型在内分泌和卵巢形态学的改变与PCOS患者相似,是较理想的PCOS模型。
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| 关 键 词： | 多囊卵巢综合征 动物模型 米非司酮 |
Study on the Mifepristone-induced animal model of polycystic ovarian syndrome in rats. |
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| Yang Zhiying,Xiao Guohong,Hou Pengzhong,et al.. Study on the Mifepristone-induced animal model of polycystic ovarian syndrome in rats.[J]. Current Advances In Obstetrics and Gynecology, 2011, 20(8): 637-640 |
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| Authors: | Yang Zhiying Xiao Guohong Hou Pengzhong et al. |
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| Affiliation: | Yang Zhiying,Xiao Guohong,Hou Pengzhong,et al.Department of Obstetrics and Gynecology,the Third Affiliated Hospital of Guangzhou Medical University,The Institute of Obstetrics and Gynecology of Guangzhou,Guangzhou 510150 |
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| Abstract: | Objective:To establish an animal model for the study of polycystic ovarian syndrome and approach its significance by using the progesterone antagonist-mifepristone.Methods:The female sprague-dawley(SD)rats with regular estrus cycle for 4 to 5 days were injected with mifepristone beginning on the first day of estrus and vaginal smears were collected daily for estrus cycle determination.After eight days,the rats and ovaries were weighed;the levels of serum LH,T,FSH,E2 and ovarian morphological changes were ex... |
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| Keywords: | Polycystic ovarian syndrome Animal model Mifepristone |
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