三氧化二砷诱导淋巴瘤Raji细胞凋亡与细胞周期阻滞

本研究探讨研究三氧化二砷（As2O3）对人淋巴瘤Raji细胞株凋亡诱导的作用特点及其机制。用MTT比色法观察不同浓度As2O3对Raji细胞的增殖抑制效应，电子显微镜观察不同浓度As2O3作用不同时间后细胞的凋亡形态，DNA-ladder琼脂糖凝胶电泳观察凋亡条带，流式细胞术检测Raji细胞凋亡、细胞周期分布。结果表明：1—8μmol／LAs2O3能明显抑制Raji细胞的活力，并存在一定的量效、时效关系。2—8μmol／L浓度的As2O3可以诱导Raji细胞周期阻滞及凋亡，而1μmol／LAs2O3不诱导细胞凋亡，只是通过细胞周期阻滞作用抑制细胞增殖。结论一定浓度三氧化二砷可以抑制Raji细胞的增殖，阻滞细胞周期，诱导细胞凋亡。细胞周期阻滞伴随细胞凋亡而发生。

Apoptosis and Cell Cycle Arrest in Lymphoma Raji Cells Induced by Arsenic Trioxide

This study was aimed to investigate the apoptosis induced by arsenic trioxide （ As2O3 ） in lymphoma Raji cells and its possible mechanisms. The inlibitory effect of different concentration of As2O3 on cell proliferation was tested by MTT assay. Apoptosis was observed with electron microscope and DNA electrophoresis. The distribution of cell cycles and cell apoptosis were detected by flow cytometry. The results showed that the 1 - 8μmol/L As2O3 inhibited Raji cell growth effectively in a dose-and time-dependent manner. As2O3 at 2 - 8 μmol/L could induce the cell apoptosis and cell cycle arrest. However, AS203 at 1 μmol/L inhibited Raji cells proliferation only by cell cycle arrest, without any signs of cell apoptosis. In conclusion, substantial proliferation inhibition, cell cycle arrest and apoptosis in Raji cells could be induced by As2O3. Cell cycle arrest happens with apoptosis, when treated with As2O3.