Penitentiary population of Mycobacterium tuberculosis in Kyrgyzstan: Exceptionally high prevalence of the Beijing genotype and its Russia-specific subtype |
| |
| Authors: | Igor Mokrousov Violeta Valcheva Nurmira Sovhozova Almaz Aldashev Nalin Rastogi Jainagul Isakova |
| |
| Affiliation: | 1. Unité de la Tuberculose et des Mycobactéries, Institut Pasteur de Guadeloupe, Abymes 97183 Guadeloupe;2. Laboratory of Molecular Microbiology, St. Petersburg Pasteur Institute, St. Petersburg 197101, Russia;3. Department of Pathogenic Bacteria, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Sofia, 1113 Bulgaria;4. Institute of Molecular Biology and Medicine, Bishkek 720040, Kyrgyzstan;1. Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran;2. Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran;1. Research Institute for Medical Genetics, Siberian Branch of Russian Academy of Medical Sciences, Tomsk, Russia;2. Siberian State Medical University, Tomsk, Russia;1. University of Iowa Hospitals and Clinics, Iowa City, Iowa;2. Department of Thoracic Surgery, Dr.-Horst-Schmidt-Klinik, Wiesbaden, Germany;3. Division of Pulmonary and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland;4. Division of Pulmonary and Critical Care Medicine, University of Maryland School of Medicine, Baltimore, Maryland;1. Laboratory of Cellular Microbiology, Oswaldo Cruz Institute (IOC), Fiocruz, Rio de Janeiro, Brazil;2. Tuberculosis Control Program and Coordination Management in Prison Health, State Department of Corrections, Rio de Janeiro, Brazil;3. Laboratory of Molecular Biology Applied to Mycobacteria, IOC, Fiocruz, Brazil;4. INSERM, UMR_S 1136, Pierre Louis Institute of Epidemiology and Public Health, Department of Social Epidemiology, France;5. Sorbonne Universités, UPMC Univ Paris 06, UMR_S 1136, Pierre Louis Institute of Epidemiology and Public Health, Department of Social Epidemiology, France;6. CRPHF/ENSP, Fiocruz, Rio de Janeiro, Brazil;7. Univ Paris-Sud, UMR8621, Orsay F-91405, France;8. CNRS, Orsay F-91405, France;9. Centre Muraz, Bobo-Dioulasso, Burkina Faso;10. Department of Epidemiology and Quantitative Methods, ENSP/Fiocruz, Rio de Janeiro, Brazil;1. Laboratory of Molecular Microbiology, St. Petersburg Pasteur Institute, St. Petersburg, Russia;2. WHO Supranational TB Reference Laboratory, Unité de la Tuberculose et des Mycobactéries, Institut Pasteur de Guadeloupe, Abymes Cedex, Guadeloupe, France;3. Institute of Molecular Biology and Medicine, Bishkek 720040, Kyrgyzstan;4. The Stephan Angeloff Institute of Microbiology BAS, Sofia, Bulgaria |
| |
| Abstract: | Here, we present results of the first study of the Mycobacterium tuberculosis genotypes circulating in Kyrgyzstan. We focused on the incarcerated population known to be at high-risk for tuberculosis (TB) and with a significant impact on TB incidence in the general population. Beijing genotype was detected in 42 of 56 M. tuberculosis sputum-extracted DNA samples from newly-diagnosed adult pulmonary TB patients. RIF and INH resistance was genotypically detected in 28% and 55% samples; 13 of 15 MDR strains belonged to Beijing genotype. 12-locus MIRU-VNTR typing showed 8 of 56 samples to be mixed cases; 7 of them contained a Beijing strain. MIRU analysis demonstrated a high homogeneity of the studied collection (HGI = 0.66) while 28 of 56 strains had a profile 223325153533 corresponding to Beijing/M2 subtype highly prevalent in different Russian settings. Three hypervariable loci, QUB-3232, VNTR-3820 and VNTR-4120, permitted to further subdivide 28 Beijing/M2 strains into 11 subtypes shared by 1 to 9 strains. To conclude, all markers taken together, the penitentiary population of M. tuberculosis in Kyrgyzstan exhibited a strong genetic affinity to Russia and a weak relatedness to East Asia. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
