阿司匹林对食管鳞癌细胞株致凋亡的作用及对烟草提取物作用的影响

目的研究非选择性环氧化酶-2(COX-2)抑制剂阿司匹林对食管癌细胞株EC109凋亡的影响,观察烟草提取物对食管癌细胞株EC109的增生作用及阿司匹林对其增生及凋亡作用的影响。方法用流式细胞仪检测细胞凋亡。噻唑蓝(MTT)比色法测定烟草提取物对食管鳞癌细胞株的增生作用。结果阿司匹林诱导EC109细胞凋亡,呈浓度时间依赖性,烟草氯仿提取物(CE)、乙醇提取物(EE)和4-(甲基亚硝胺)-1-(3-吡啶基)-1-丁酮(NNK)在一定浓度范围内对食管鳞癌细胞株EC109有促增生作用。阿司匹林可以抑制烟草的促增生作用。一定浓度范围内的CE和NNK可以抑制阿司匹林4 mmol/L诱导凋亡的作用,而EE无此作用。结论阿司匹林通过诱导EC109细胞发生凋亡抑制细胞生长,其致凋亡作用可被烟草所抑制,而烟草的促食管癌细胞增生作用可能与COX-2途径有关。

Effects of Aspirin on Apoptosis and Effects of Cigarette Extracts on Proliferation and Apoptosis in Esophageal Squamous Cell Carcinomas Cell Lines

Objective To verify the hypothesis that aspirin,a non-selective COX-2 inhibitor,could induce apoptosis in ESCC cell lines and to evaluate the effects of cigarette smoking extracts on proliferation and apoptosis in ESCC cell lines.In addition,it is developed that the interaction between cigarette smoke extracts and NSAIDs on ESCC cell lines.Methods The apoptosis was determined by flow cytometry.MTT assay was performed to study the effect of cigarette smoking extracts on esophageal cancer cell growth.Results Aspirin was found to be able to induce the apoptosis of EC109 cell,and the apoptosis was dose and time dependent.Results showed that the proliferation of EC109 cell lines were promoted by CE(1～40 mg/L),EE(10～80 mg/L) and NNK(0.01～10 mmol/L) in 24 h.The proliferation of cell lines were inhibited by aspirin.Both CE and NNK could inhibit the apoptosis that was induced by aspirin.EE has no similar effects on the apoptosis.Conclusion Aspirin could inhibit the proliferation of EC109 cell by inducing the apoptosis,cigarette smoking extracts could inhibit the apoptosis that was induced by aspirin.It is suggested that the effects of cigarette smoking extracts on cell proliferation is related to COX-2.