| 羊栖菜中清除自由基活性物质的研究 |
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| 引用本文： | 韩华,战松梅,单联刚,王春波. 羊栖菜中清除自由基活性物质的研究[J]. 中国海洋药物, 2008, 27(3) |
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| 作者姓名： | 韩华 战松梅 单联刚 王春波 |
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| 作者单位： | 1. 上海水产大学食品学院,上海,200090
2. 青岛大学医学院,山东,青岛,266071 |
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| 摘 要： | 目的 通过不同溶剂对羊栖菜的有效成分进行提取和体外活性检测,以期筛选出具有一定开发潜力的自由基清除荆.方法 采用DPPH(二苯代苦味酰自由基)体系、超氧阴离子体系对不同提取物进行清除自由基活性测定,并与海带、紫菜的提取物及抗坏血酸(Vit C)和荼多酚作活性对照.结果 不同溶剂对羊栖菜的提取率不同,各种提取物之间的清除自由基活性存在一定差异.结论 0.5%Na2CO3溶液提取率最高,且提取物清除自由基的能力最强,以DPPH体系作为检测系统时测量结果较为稳定,效果也较明显.
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| 关 键 词： | 羊栖菜 自由基 DPPH体系 多酚氧化酶体系 |
Studies on the free radical scavenging activity of bioactive sub-stances from Sargassum fusiforme |
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| HAN Hu,ZHAN Song-mei,SHAN Lian-gang,WANG Chun-bo. Studies on the free radical scavenging activity of bioactive sub-stances from Sargassum fusiforme[J]. Chinese Journal of Marine Drugs, 2008, 27(3) |
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| Authors: | HAN Hu ZHAN Song-mei SHAN Lian-gang WANG Chun-bo |
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| Affiliation: | HAN Hua1,ZHAN Song-mei2,SHAN Lian-gang1,WANG Chun-bo2(1.Bromatology Institute of Shanghai Fisheries University,Shanghai 200090,China,2.Medical College of Qingdao University,Qingdao 266071,China) |
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| Abstract: | Objective Bioactive components were extracted from Sargassum fusiforme by using different solvents and their activities were detected in vitro in order to screen potential free radical scavenger.Methods Using the assay system of DPPH,superoxide radical,and in comparison with vitamin C,tea polyphenols and a series of extracts of sea tangle,laver.Results Different solvents had different extraction rates,and extracts by different solvents had different free radical scavenging activities.Conclusion 0.5%Na2CO3 s... |
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| Keywords: | Sargassum fusiforme(Harv.)Setch free radical DPPH system polyphenol oxidase(PPO)system |
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