Box-behnken响应面法优化紫红生肌软膏的醇提工艺

目的 优选紫红生肌软膏的醇提工艺参数,为紫红生肌软膏的新药开发提供依据.方法 以栀子苷、连翘酯苷A、羟基红花黄色素A为评价指标,在单因素试验的基础上,以乙醇浓度、加醇量、提取时间为考察因素,采用Box-behnken响应面法设计试验,采用层次分析法-熵权法组合赋权确定各指标权重系数并计算综合评分,从而优化紫红生肌软膏醇...

Optimization of Ethanol-extraction Process of Zihong Shengji Ointment Based on Box-behnken Response Surface Design

Objective To optimize the alcohol-extraction process parameters of Zihong Shengji Ointment and provide the basis for the new drug development of Zihong Shengji Ointment.Methods The geniposide,forsythiaside A,hydroxysafflor yellow A were used as evaluation indexes.On the basis of single factor experiment,the ethanol concentration,the amount of alcohol added and extraction time were used as investigation factors,the experiments were designed by Box-behnken response surface,and the weight coefficients of each index were determined by analytic hierarchy process-information entropy weight method,and the comprehensive score was calculated,so as to optimize the alcohol-extraction process parameters of Zihong Shengji Ointment.Results The optimum alcohol-extraction process of Zihong Shengji Ointment was 70%ethanol for two times,the first time was added with 9 times ethanol for 150 min,and the second time was added with 7 times ethanol for 120 min.The measured value of the comprehensive score of the process parameters was 100.70,which was close to the predicted value.Conclusion The optimized alcohol-extraction process of Zihong Shengji Ointment is stable and feasible,which can provide basis for the further development of Zihong Shengji Ointment as new drugs.