枸杞子-丹参对小鼠视网膜Caspase-12前体、Caspase-2表达的影响

目的观察枸杞子-丹参对rd10小鼠视网膜Caspase-12前体、Caspase-2表达的影响。方法将40只rd10小鼠随机分为5组,空白组灌胃生理盐水;对照组灌胃维生素A溶剂;枸杞组灌胃枸杞子中药超微配方颗粒溶液;丹参组灌胃丹参中药超微配方颗粒溶液;枸杞加丹参组(杞参组)灌胃枸杞子加丹参中药超微配方颗粒溶液;8只C57小鼠为野生组,灌胃生理盐水。给药28 d后,RT-qPCR检测小鼠视网膜Caspase-12前体、Caspase-2 mRNA表达情况,免疫组化、免疫荧光法检测小鼠视网膜Caspase-12前体、Caspase-2蛋白表达情况。结果 RT-qPCR检测显示:除杞参组外,各组小鼠视网膜Caspase-12前体、Caspase-2 mRNA相对表达量高于野生组,差异有统计学意义(P<0.05);杞参组小鼠视网膜Caspase-12前体、Caspase-2 mRNA相对表达量低于空白组、对照组,差异均有统计学意义(P<0.05)。免疫组化检测显示:各组小鼠视网膜Caspase-12前体、Caspase-2平均光密度高于野生组,差异均有统计学意义(P<0.05);杞参组Caspase-12前体、Caspase-2蛋白平均光密度低于空白组、对照组、枸杞组、丹参组,差异均有统计学意义(P<0.05)。免疫荧光检测显示:各组小鼠视网膜Caspase-12前体、Caspase-2蛋白平均荧光强度高于野生组,差异均有统计学意义(P<0.05);杞参组Caspase-12前体、Caspase-2蛋白平均荧光强度低于空白组、对照组、枸杞组、丹参组,差异均有统计学意义(P<0.05)。结论枸杞子-丹参可以抑制rd10小鼠视网膜Caspase-12前体、Caspase-2 mRNA和蛋白的表达,从而抑制感光细胞的凋亡,维持视网膜正常功能结构,保护视功能。

Effect of Gouqizi(Lycii Fructus)and Danshen(Salviae Miltiorrhizae Radix Et Rhizoma)on the Expression of Caspase-12 Precursor and Caspase-2 in Retinal of rd10 Mice

Objective To observe the effect of Gouqizi(Lycii Fructus)and Danshen(Salviae Miltiorrhizae Radix Et Rhizoma)on the expression of Caspase-12 precursor and Caspase-2 in rd10 mice.Methods 40 rd10 mice were randomly divided into 5 groups.The blank group was given distilled water;the control group was given intragastric vitamin A solvent;the Gouqizi(LyciiFructus)group was given intragastric Gouqizi(Lycii Fructus)Chinese medicine ultrafine granular solution;the Danshen(Salviae Miltiorrhizae Radix Et Rhizoma)group was given intragastric Danshen(Salviae Miltiorrhizae Radix Et Rhizoma)Chinese medicine ultrafine formula granule solution;Gouqizi(Lycii Fructus)plus Danshen(Salviae Miltiorrhizae Radix Et Rhizoma)group(QiShen group)was given intragastric Gouqizi(Lycii Fructus)plus Danshen(Salviae Miltiorrhizae Radix Et Rhizoma)Chinese medicine superfine granule solution;8 C57 mice as the wild group,was given intragastric distilled water.28 days after the administration,RT-qPCR was used to detect the expression of Caspase-12 precursor and Caspase-2 mRNA in the retina of rd10 mice,and the expression of Caspase-12 precursor and Caspase-2 proteins in the retina was detected by immunohistochemistry and immunofluor-escence.Results The RT-qPCR test showed that except for the QiShen group,the relative expression levels of Caspase-12 precursor and Caspase-2 mRNA in the retina of each group were higher than that of the wild group,and the differences were statistically significant(P<0.05);the relative expression levels of Caspase-12 precursor and Caspase-2 mRNA in the retina of the QiShen group were lower than that of the blank group and the control group,the differences were statistically significant(P<0.05).Immunohistochemical detection showed that the average optical density of Caspase-12 precursor and Caspase-2 in the retina of mice in each group was higher than that of the wild group,and the differences were statistically significant(P<0.05);the average optical density of Caspase-12 precursor and Caspase-2 of the QiShen group was lower than that of the blank group,control group,Gouqizi(Lycii Fructus)group,and Danshen(Salviae Miltiorrhizae Radix Et Rhizoma)group,and the differences were statistically significant(P<0.05).Immunofluorescence detection showed that the average fluorescence intensity of Caspase-12 precursor and Caspase-2 in the retina of mice in each group was higher than that in the wild group,and the differences were statistically significant(P<0.05);Caspase-12 precursor and Caspase-2 average fluorescence intensity of QiShen group was lower than that of the blank group,control group,Gouqizi(Lycii Fructus)group and Danshen(Salviae Miltiorrhizae Radix Et Rhizoma)group,and the differences were statistically significant(P<0.05).Conclusion Gouqizi(Lycii Fructus)and Danshen(Salviae Miltiorrhizae Radix Et Rhizoma)can inhibit the expression of rd10 mice Caspase-12 precursors,Caspase-2,thereby inhibiting the apoptosis of photoreceptor cells,maintaining normal functional structure of retina,protecting view function.