HPLC-ECD法同时测定海洛因急性中毒大鼠不同脑区 中单胺类神经递质含量

目的:建立高效液相色谱-电化学检测器(HPLC-ECD)法同时测定大鼠伏隔核和纹状体两个不同脑区中单胺类神经递质多巴胺(DA)和5-羟色胺(5-HT)含量的方法,并对海洛因急性中毒大鼠不同脑区中单胺类神经递质的含量变化进行研究.方法:高效液相色谱采用QUATTRO C18色谱柱,以磷酸二氢钠-柠檬酸-辛烷磺酸钠缓冲液:...

Simultaneous Determination of Monoamine Neurotransmitters in Different Brain Regions of Rats With Acute heroin Poisoning by HPLC-ECD

Objective:To establish a HPLC-ECD method to simultaneously detect the contents of monoamine neurotransmitters dopamine(DA)and serotonin(5-HT)in the nucleus accumbens and striatum of rats and apply it to study the content changes of DA and 5-HT in different brain areas of rats with heroin acute poisoning.Methods:HPLC adopted QUATTRO C18 column,and used sodium dihydrogen phosphate-citric acid-sodium octane sulfonate buffer∶acetonitrile(94∶6)as the mobile phase,the flow rate was 0.3 ml/min,and the injection volume was 20μl.The specificity,linear range,precision,recovery and stability of this method were investigated and the contents of DA and 5-HT in the nucleus accumbens and striatum of rats with acute heroin poisoning were measured.Results:The detection limit and quantitative limit of DA and 5-HT were both 0.2 ng/ml and 0.5 ng/ml,respectively.Intraday and interday precisions(RSDs)were less than 5%;The recovery range was 87.8% to 115.5%;The brain tissue samples were stable after being placed in 4℃ for 4 d.After administration of acute toxic doses of heroin,the DA contents in the two brain regions of the rats were basically unchanged,but the 5-HT contents increased significantly in a dose-dependent manner.Conclusion:The method is accurate,sensitive and stable,and is suitable for the determination of DA and 5-HT contents in different brain regions of rats.It was first discovered that the acute toxicity of heroin may be related to the significant increase of 5-HT contents in the nucleus accumbens and striatum.The specific mechanism needs to be further studied and verified.