不明原因发热患者肺泡灌洗液病毒群落解析

目的: 分析不明原因发热患者肺泡灌洗液中的病毒谱。方法: 将58例肺泡灌洗液样本随机分为5组分别构建5个文库,利用病毒宏基因组学技术进行病毒群落分析,并对获得的细环病毒及鼻病毒进行阳性样本筛选。结果： 肺泡灌洗液病毒群落组成中,指环病毒科占主体地位（19.4%）,其次为圆环病毒科（11.0%）,藻去氧核糖核酸病毒科（11.0%）,人类内源性递转录病毒科（10.2%）,痘病毒科（4.9%）,逆转录病毒科（3.2%）,帚状病毒科（2.3%）,小RNA病毒科（0.6%）;并发现了4株序列较长的细环病毒,根据细环病毒开放阅读框1进行系统进化分析,显示其与已知的细环病毒在核酸水平上有一定的差异。通过对细环病毒及鼻病毒阳性样本的筛选,发现细环病毒阳性率为11.3%,鼻病毒阳性率为10.0%。结论： 应用病毒宏基因组学技术成功解析出不明原因发热患者肺泡灌洗液中的病毒群落组成,病毒群落中指环病毒科占比最高,其中细环病毒因发热导致的炎性环境以及患者免疫力低下而大量增殖。

Analysis of viral community in bronchoalveolar lavage fluid of patients with fever of unknown origin

Objective: To analyze the virus profile in alveolar lavage fluid of patients with fever of unknown origin. Methods: A total of 58 samples of alveolar lavage fluid were randomly divided into five groups to construct 5 libraries.  Virus metagenomics technology was used to analyze the virus community of five libraries, and PCR screening was performed for the obtained torque teno virus and rhinovirus. Results: The results of virus composition showed that the virus community was dominated by Anelloviridae, which accounted for 19.4% of the total population, followed by Circoviridae(11.0%), Phycodnaviridae(11.0%), Human endogenous retrovirus(10.2%), Poxviridae(4.9%), Retroviridae(3.2%),Virgaviridae(2.3%) and Picornaviridae(0.6%). Four strains of torque teno virus with long sequences were found, and phylogenetic analysis was carried out according to open reading frame 1 of torque teno virus, which showed that it was different from the known virus in some sequences. The positive rate of torque teno virus and rhinovirus was 11.3% and 10.0% respectively by PCR screening. Conclusion: The viral community composition in alveolar lavage fluid of patients with fever of unknown origin was determined with virus metagenomics technology. The Anelloviridae had the highest proportion in the virus community, in which the torque teno virus proliferated due to the inflammatory environment caused by fever and the low immunity of patients.