血管移植后血管平滑肌细胞PCNA表达及药物对其影响

目的：探讨Ｂａｔｒｏｘｏｂｉｎ等药物对兔静脉移植模型早期ＰＣＮＡ表达的影响。方法：兔颈静脉移植于颈动脉后应用Ｂａｔｒｏｘｂｉｎ，ＬＭＷＨ，Ｕｒｏｋｉｎａｓｅ和Ｖｅｒａｐａｍｉｌ治疗，并于不同时间采用免疫组化方法观察血管平滑肌（ＶＳＭＣ）中ＰＣＮＡ表达的变化。结果：术后３、７，１４ｄ均有ＰＣＮＡ的表达；３ｄ的Ｕｒｏｋｉｎａｓｅ，ＬＭＷＨ两组ＰＣＮＡ的表达与对照组无显著差异；Ｖｅｒａｐａｍｉｌ及Ｂａｔ

The Effect of Proliferating Cell Nuclear Antigen Expression in Vein Grafts and Join Arteries of Rabbits by Drugs

Objective: To investigate the influence of Batroxobin and other drugs on expression of proliferating cell nuclear antigen in early transplant vessels of Newzealand rabbit model with jugular vein transplanted to carotid. Methods: With the techniques of pathological, immunohistochemical to detect the expression of vascular smooth muscle cell in vein grafts and join arteries. Results: There was rather active PCNA expression on 3 d, 7 d and 14 d after venous graft, especially for 3 d. Urokinase and LMWH had no effect on PCNA expression in VSMC, but there was a significant inhibitory effect in Batroxobin and Verapamil groups compared with the three groups of contrast, Urodinase and LMWH. Conclusions: Batroxobin and Verapamil can inhibit the PCNA expression of vein grafts and join arteries in different time.