Molecular mechanism of SSFA2 deletion inhibiting cell proliferation and promoting cell apoptosis in glioma |
| |
Authors: | Aihua Zhu Xuetao Li Haibin Wu Zongning Miao Fenglai Yuan Feng Zhang Bei Wang Youxin Zhou |
| |
Institution: | 1. Department of Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, 899 Pinghai Street, Suzhou, 215006, Jiangsu, China;2. The Research Institute of Wuxi Third People’s Hospital, The Third Affiliated Hospital of Nantong University, 585 Xingyuan North Road, Wuxi, 214041, Jiangsu, China;3. Department of Neurosurgery of Wuxi Third People’s Hospital Research, The Third Affiliated Hospital of Nantong University, 585 Xingyuan North Road, Wuxi, 214041, Jiangsu, China |
| |
Abstract: | Gliomas are the most common primary brain malignant tumors in humans. Glioblastoma multiforme(GBM) is the most malignant intracranial tumor with a relatively poor prognosis. There promote us to find effective anti-cancer therapies to reduce cancer mortality. By using bioinformatic analysis, we found SSFA2 as a gene with elevated expression in the glioma tissues. We detected the expression of SSFA2 in glioma tissues and in the glioma cell lines, as well as in normal brain tissues. SSFA2 expression was higher in glioma tissues, especially in glioblastoma multiforme than normal brain tissues. Subsequently, we found that down-regulate SSFA2 in glioma cell lines can regulate the cell cycle to reduce the proliferation ability and induce the early apoptosis rate in shSSFA2 cells relative to control cells. Moreover, we found that down-regulate SSFA2 in glioma cell line U87(shSSFA2-U87) inhibited the growth effectiveness compared to the control cell line U87. These result reveals us that SSFA2 may act as oncogene to promote the progression of glioma. For further research specific mechanisms of SSFA2 in gliomas, we used the gene chip to detect the downstream gene in U87. We found that 30 genes also may be as target gene of SSFA2, and we testify the protein expression by western-blot. The result reveal that IL1A, IL1B and CDK6 as target gene of SSFA2 to regulate the progression of glioma. These finding suggest that SSFA2 could be a new therapeutic target for gliomas. |
| |
Keywords: | SSFA2 Glioma Cell proliferation Cell cycle Apoptosis |
本文献已被 ScienceDirect 等数据库收录! |
|