| 人透明带蛋白3重组表达载体的构建与鉴定 |
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| 引用本文： | 张家颖,王弘珺,左文静,王忠山. 人透明带蛋白3重组表达载体的构建与鉴定[J]. 吉林大学学报(医学版), 2004, 30(5): 681-683. DOI: 国家自然科学基金资助课题 |
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| 作者姓名： | 张家颖 王弘珺 左文静 王忠山 |
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| 作者单位： | 1. 吉林大学基础医学院生物化学与分子生物学实验中心，吉林 长春130021；2. 吉林大学基础医学院细胞生物学教研室，吉林 长春130021 |
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| 摘 要： | 目的：构建高效表达人透明带蛋白3的真核重组表达载体。方法：利用PCR、T-A载体克隆和亚克隆等技术。结果：构建成pGEM-ZP3重组质粒及pcDNA3.1ZP3dhfr真核重组表达载体。结论：经酶切鉴定构建的人透明带蛋白3重组表达载体可高效表达重组人ZP3蛋白。
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| 关 键 词： | 透明带蛋白3 质粒 |
| 文章编号： | 1671-587X(2004)05-0681-03 |
| 收稿时间： | 2004-02-20 |
| 修稿时间： | 2004-02-20 |
Construction and identification of recombinant human zona pellucida 3 expression vector |
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| ZHANG Jia-ying,WANG Hong-jun,ZUO Wen-jing,WANG Zhong-shan. Construction and identification of recombinant human zona pellucida 3 expression vector[J]. Journal of Jilin University: Med Ed, 2004, 30(5): 681-683. DOI: 国家自然科学基金资助课题 |
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| Authors: | ZHANG Jia-ying WANG Hong-jun ZUO Wen-jing WANG Zhong-shan |
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| Affiliation: | 1. Experimental Center of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Jilin University, Changchun 130021,China;2. Department of Cell Biology, Schoolof Basic Medical Sciences, Jilin University， Changchun 130021,China |
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| Abstract: | Objective To construct the recombinant plasmid that highly expressed human zona pellucida 3. Methods The techniques of PCR amplification, T-A vector ligation, and sub-clone were used. Results The pGEM-TZP3 plasmid and pcDNA3.1ZP3dhfr recombinant expression vector were successfully constructed. Conclusion The recombinant expression plasmid constructed by restriction enzyme cleave identification can highly express recombinant human ZP3 protein. |
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| Keywords: | gene recombination zona pellucida 3 plasmid |
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