Co-culture of early embryo with human decidual stromal cellsin vitro by improvement of early embryo development |
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Authors: | Yan Jie Zhu Guijin Liu Jianxin Ai Jihui |
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Institution: | (1) Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical University, 430030 Wuhan |
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Abstract: | Summary An early embryo co-culture system with human decidual stromal cells was established to study its effect on early embryonic
cleavage and growth in vitro. Three hundred and eight 2-cell mouse embryos were co-cultured with human decidual stromal cell
monolayer in MEM + 0.4 % bovine serum albumin (BSA) and 163 embryos cultured in MEM + 15 % FCS alone as control. Among the
mouse 2-cell embryos co-cultured with human decidual stromal cells, 72.73 % developed to the morula stage and 67.21 % cavitated
to blastocysts with 59.74 % hatching, as compared with 61.34 % to morula stage, 48.47 % to blastocysts and none hatching in
the controls, respectively. Co-cultured embryos cleaved slightly faster than controls and showed no or less fragmentation
than those in the control. These results suggested that human decidual stromal cells can support early embryonic development
and yield a reasonable number of embryos with good quality up to blastocyst stage. |
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Keywords: | human decidual stromal cells embryo co-culture |
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