原发性胆汁性肝硬化患者外周血单个核细胞Toll样受体表达与调节性T细胞相关性分析

目的 探讨原发性胆汁性肝硬化(PBC)患者外周血单个核细胞(PBMC)Toll样受体2、4、9(TLR2,TLR4,TLR9)表达及PBMC中CD4+ CD25+调节性T细胞(Tregs)特点和影响因素.方法 采用流式细胞术检测PBC患者(52例)和健康对照者(22例)外周血PBMC中TLR2、4、9阳性细胞及Tregs比例,比较其在PBC患者和健康对照者中的差别并分析TLR与Tregs相关性.结果 PBC患者Tregs比例低于健康对照者(1.53+1.33)vs(4.42±1.43),P＜0.0001],TLR4阳性细胞比例高于健康对照者(36.95±3.53)vs(32.84±8.06),P=0.003]；PBC患者Tregs比例与TLR9阳性细胞比例呈负相关(R2 =0.115,P=0.016),健康对照者Tregs比例与TLR2,4和TLR2,4,9联合表达阳性细胞比例呈负相关(R2=0.326,P=0.007；R2 =0.226,P=0.034).结论 PBC患者外周血PBMC中Tregs比例降低,TLR4表达升高,可能受肝硬化失代偿期腹腔感染的影响.

Expression of Toll like receptors on peripheral blood mononuclear cells and their influence on CD4~+CD25~+T regulatory cells in patients with primary biliary cirrhosis

Objective To investigate the expression of toll like receptors(TLR) on peripheral blood mononuclear cells(PBMC) and CD4+CD25+T regulatory cells in patients with primary biliary cirrhosis(PBC).Methods We analysed TLR2,4,9 on PBMC and CD4+CD25+Tregs of 52 PBC patients and 22 normal controls using flow cytometry.We compared the parameters between patients and normal controls.Results CD4+CD25+T regulatory cells in PBC patients were lower than that of normal controls (1.53±1.33) vs(4.42±1.43),P<0.0001],however,TLR4 cells in PBC patients were higher than that of normal controls (36.95±3.53) vs(32.84±8.06),P=0.003].Tregs and TLR9 cells were negatively correlated in PBC patients(R2=0.115,P=0.016),whereas only Tregs were negatively correlated with TLR2,4/TLR2,4,9 cells in normal controls(R2=0.326,P=0.007;R2=0.226,P=0.034).Conclusion Tregs are defective numerically in PBC patients and the expression of TLR4 cells is enhanced,especially in patients with infection.Tregs may affect numerically by infection,while they are defective numerically in PBC patients.