散发性结直肠癌中微卫星不稳定性研究

目的：检测微卫星不稳定性（Microsatellte Instability,MIN)在国内散发性结直肠癌中的发性情况并探讨其与散发性结直肠癌临床病理参数之关系。方法：彩和PCR，变性聚丙烯酰胺凝胶电泳，银染色显色等技术，选用位于4条染色体上的6个不同的微卫星位点，对60例散发性结直肠癌病例进行了微卫星不稳定性检测。结果：51．67％（31／60）的病例在1个或1个以上位点出现MIN，33．33％（20／60）的病例在2个或2个以上位点出现MIN，即RER＋（Replication ERRORS pOSITIVE)。各位点在散发性结肠癌中的检出率不同，其顺序为P53（1），D18S363，NF1，D18S46，ANK1，APC（1）。在临病理参数上，RER＋组和RER－组相比在肿瘤发生部位，组织学分化、浸润深度，肿瘤大小等方面具有不同的趋势，但没有显著性差异；而在局部淋巴结转移上具有显著性差异（P＜0．05）。结论：散发性结直肠癌中广泛存在着MIN；RER＋结直肠癌和RER－结直肠癌在临床病理参数上有着不同的趋势。

Microsatellite Instability in Sporadic Colorectal Carcinoma

Objective: The aim of this study was to detect the microsatellite instability (MIN) status in sporadic colorectal carcinoma (CRC) in Chinese population and to compare the MIN status of CRC with its clinicopathological parameters. Methods: In current study, 6 microsatellite loci from 4 different chromosomes were detected by PCR, denatured-polyacrylamide gel electrophoresis and silver-staining methods. Results: 51.67% (31/60) of the cases showed MIN-positive at one or more than one locus, and 33.33% (20/60) of the cases did MIN-positive at two or more than two loci, so called PER-positive. All the 6 loci showed MIN at different sensitivity. The order was: p53 (l) was the most sensitive, then the D185363, NF1, D18546, ANK1, and the least sensitive APC (l). By comparing the RER-positive cases with the RER-negative ones, RER-positive group had some different tendency from the RER-negative group, including the tumor size, tumor localization, invasive depth of the tumor and differentiation, although there was no statistic significance. The metastasis fate of RER-positive group was lower than that of the RER-negative group and had statistic significance (P<0.05). Conclusion: MIN phenotype is present ubiquitously in sporadic CRC in Chinese population and RER-positive CRC has some clinicopathological features different from that of RER-negative group.