Th22细胞及相关因子在重症肌无力患者外周血中的表达

目的研究重症肌无力(myasthenia gravis,MG)患者外周血辅助性T细胞22(T helper 22cells,Th22)和白细胞介素-22(interleukin-22,IL-22)的表达以及两者间的相关性。方法收集25例MG患者和24例健康对照者,其中眼肌型重症肌无力(ocular myasthenia gravis,OMG)患者14例,全身型重症肌无力(general myasthenia gravis,GMG)患者11例。采用流式细胞仪检测MG患者和健康对照者外周血单个核细胞(peripheral blood mononuclear cells,PBMC)中Th22细胞的比例,采用酶联免疫吸附实验(enzyme-linked immunosorbent assay,ELISA)检测血浆IL-22的表达。比较各组间Th22细胞比例和IL-22表达水平差异,以及Th22细胞比例和IL-22表达间的相关性。结果 MG患者PBMC中Th22细胞比例、血浆IL-22表达水平均显著低于健康对照组(0.60±0.07)%比(0.92±0.09)%,P0.01;(18.65±1.38)pg/mL比(24.54±1.85)pg/mL,P0.05];OMG与GMG患者间Th22细胞比例、IL-22表达水平均无统计学差异(均P0.05);MG患者PBMC中Th22细胞比例与IL-22表达水平间呈中度正相关(r=0.59,P0.01)。结论 MG患者体内Th22细胞比例及血浆IL-22表达水平减低可能导致免疫功能紊乱进而影响MG的发病。

Changes of circulating T helper 22 cells and its related molecules in myasthenia gravis

Objective To study the expression of T helper 22 (Th22) cells and interleukin-22 (IL-22) in peripheral blood of patients with myasthenia gravis (MG);the correlation between Th22 cells and IL-22 was analyzed.Methods Twenty-five MG patients,consisting of fourteen ocular MG (OMG) patients and eleven general MG patients (GMG),were enrolled.Twenty-four healthy volunteers were used as controls.The percentages of Th22 cells in peripheral blood mononuclear cells (PBMC) of MG patients and healthy controls were detected by flow cytometry.The plasma levels of IL-22 were measured by enzyme linked immunosorbent assay (ELISA).The differences of the Th22 cells percentages and the concentration of IL-22 were compared between each group;also we investigated the correlation between Th22 cells and IL-22.Results The proportion of Th22 cells and plasma IL-22 levels significantly declined in MG patients compared with healthy controls (0.60±0.07)％ vs.(0.92±0.09)％,P＜0.01;(18.65±1.38) pg/mLvs.(24.54±1.85) pg/mL,P＜0.05].There were no significant differences of the proportion of Th22 cells and plasma IL-22 levels between OMG and GMG patients (all P＞0.05).Moderate positive correlation was shown between the proportion of Th22 cells and plasma IL-22 levels in PBMC of MG patients (r=0.59,P＜0.01).Conclusions The decreased Th22 cells and IL-22 in MG patients might lead to immune disorder,and then were involved in the pathological process of MG.