福建省肺非结核分枝杆菌临床分离株的菌种鉴定

目的 对2005-2011年间,福建省福州市胸科医院分离获得的非结核分枝杆菌临床菌株进行菌种鉴定.方法 分别使用传统鉴别培养基法和多位点PCR、hsp65-PRA与rpoB-PRA,以及hsp65、rpoB、16s rRNA和ITS基因测序等分子生物学方法,对全部菌株进行菌种鉴定.结果 450株被初步鉴定为非结核分枝杆菌的临床分离菌株中,有45株被鉴定为结核分枝杆菌,其余405株被鉴定为23种不同的非结核分枝杆菌和1株支气管戈登菌.结论 在该地区有多种非结核分枝杆菌传播和流行,其中,6种非结核分枝杆菌和支气管戈登菌为在福建省内首次发现.

Identification of non-tuberculosis mycobacteria species of clinical isolates from patients clinically diagnosed with tuberculosis in Fujian Province,China

Some species of non-tuberculosis mycobacteria (NTM are important causes of human disease and infections.The purpose of this study was to describe the NTM species of clinical isolates from patients with tuberculosis in Fujian,China.The clinical NTM strains isolated from the patients clinically diagnosed with tuberculosis collected from 2005 to 2011 in Fuzhou Pulmonary Hospital,Fujian Province,and were identified to species level using conventional and molecular methods including multi-loci PCR,PRA-hsp65 and PRA-rpoB and sequencing for hsp65,rpoB,16S rRNA and ITS.A total of 450 pre-identified NTM isolates were identified as 45 (10.00％) Mycobacterium tuberculosis strains and 405 (90.00 ％) NTM strains,which including 23 different NTM species and a Gordonia bronchialis isolates.There were at least 23 NTM species as potential pathogens were found to cause pulmonary infections in human beings,and 6 NTM species and Gordonia bronchialis were firstly identified in patients with a clinical diagnosis of tuberculosis (TB) in Fujian Province.NTM species identification is very important for distinguishing between TB and NTM pulmonary diseases.And combination of several methods can increase identification accuracy and efficacy.