Inhibition of cerebral ischemia/reperfusion injury-induced apoptosis:nicotilforin and JAK2/STAT3 pathway |
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Authors: | Guang-qiang Hu Xi Du Yong-jie Li Xiao-qing Gao Bi-qiong Chen Lu Yu |
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Institution: | 1. Department of Anatomy, Southwest Medical University, Luzhou, Sichuan Province, China;2. Department of Chemistry, Southwest Medical University, Luzhou, Sichuan Province, China;3. Drug Discovery Research Center, Southwest Medical University, Luzhou, Sichuan Province, China;4. Department of Anatomy and Neurobiology, Southwest Medical University, Luzhou, Sichuan Province, China |
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Abstract: | Nicotilforin is a lfavonoid extracted from Carthamus tinctorius. Previous studies have shown its cerebral protective effect, but the mecha-nism is undeifned. In this study, we aimed to determine whether nicotilforin protects against cerebral ischemia/reperfusion injury-induced apoptosis through the JAK2/STAT3 pathway. hTe cerebral ischemia/reperfusion injury model was established by middle cerebral artery occlusion/reperfusion. Nicotilforin (10 mg/kg) was administered by tail vein injection. Cell apoptosis in the ischemic cerebral cortex was examined by hematoxylin-eosin staining and terminal deoxynucleotidyl transferase dUTP nick end labeling assay. Bcl-2 and Bax expres-sion levels in ischemic cerebral cortex were examined by immunohistochemial staining. Additionally, p-JAK2, p-STAT3, Bcl-2, Bax, and caspase-3 levels in ischemic cerebral cortex were examined by western blot assay. Nicotilforin altered the shape and structure of injured neurons, decreased the number of apoptotic cells, down-regulates expression of p-JAK2, p-STAT3, caspase-3, and Bax, decreased Bax immunoredactivity, and increased Bcl-2 protein expression and immunoreactivity. hTese results suggest that nicotilforin protects against cerebral ischemia/reperfusion injury-induced apoptosis via the JAK2/STAT3 pathway. |
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Keywords: | nerve regeneration brain injury nicotilforin ischemic stroke cerebral ischemia/reperfusion injury treatment cell apoptosis terminal deoxynucleotidyl transferase dUTP nick end labeling JAK2/STAT3 pathway Bcl-2 Bax caspase-3 neural regeneration |
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