人食物中毒、屠宰及市售禽肉样品中沙门菌毒力基因结果分析

目的了解石家庄地区不同来源沙门菌毒力基因携带状况,为进一步开展沙门菌风险评估提供基础数据。方法收集石家庄地区2011年至2016年食物中毒、屠宰场鸡肉及市售鸡肉的沙门菌分离株186株,进行血清分型,对8种毒力基因(invA、sopE、agfA、spvR、hilA、stn、pefA、shdA)进行PCR检测。结果石家庄地区不同来源沙门菌中,共检出13种血清型,以肠炎沙门菌为显著优势群,上述8种毒力基因均有检出,其中携带率较高的毒力基因为hilA、stn、invA,分别是90.3%(168/186)、86.6%(161/186)和82.8%(154/186)。结论不同来源沙门菌的毒力基因携带状况不同。生禽食品的终端销售环节出现了较严重的污染,应采取措施加强对石家庄地区各早市流动生禽摊点储存和销售环节的食品卫生监督与防控。

Salmonella virulence gene analysis in poisoning food,slaughtering and commercial samples

We investigated the carrying status of the virulence genes of Salmonella from different sources in Shijiazhuang City,China,to provide the basic data for the further risk assessment of Salmonella.A total of 186 isolates of Salmonella from different sources were collected and identified serotypes in the area of Shijiazhuang from 2011 to 2016.PCR was performed for eight virulence genes (invA,sopE,agfA,spvR,hilA,stn,pefA,shdA).These Salmonella bacteria were detected in 13 kinds of serotypes.Enteritidis is a significant advantage of the group.The above 8 virulence genes were analyzed,and the virulence genes hilA,stn and invA were the most frequently carried,their respective carrying rate were 90.3％ (168/186),86.6％ (161/186) and 82.8％ (154/186) respectively.We found the virulence genes of Salmonella from different sources were different.It is necessary to take measures to strengthen the food hygiene supervision and prevention and control of the storage and sale of raw poultry stalls in the morning market in Shijiazhuang area.