吉林省鸽子鹦鹉热衣原体的分子流行病学调查和基因型分布研究

目的 2015年3-5月调查吉林省长春市和吉林市肉鸽与信鸽中鹦鹉热衣原体的流行情况及基因型分布.方法 本研究共采集鸽子粪便样本399份,其中长春市样本282份,吉林市样本117份.利用PCR技术进行鹦鹉热衣原体ompA基因扩增、测序以及基因型分析.结果 本实验结果显示:鹦鹉热衣原体的感染率为5.01％(21/399),其中吉林市鹦鹉热衣原体的感染率(9.40％)明显高于长春市的感染率(3.19％).此外,品种也是与衣原体感染相关的主要风险因素,肉鸽的感染率为7.49％,而信鸽的感染率为0.ompA基因的序列分析显示,这些鹦鹉热衣原体都属于B型.结论 综上所述,我国吉林省肉鸽具有较高的B型鹦鹉热衣原体流行,给人类的健康带来了潜在的威胁.

Prevalence and genotypes of Chlamydia psittaci in pigeons in Jilin Province,Northeastern China

Chlamydia psittaci is a causative agent of psittacosis,which can infect a wide range of hosts including birds and humans.However,information regarding C.psittaci infection in pigeons is scarce.In the present study,a total of 399 fecal samples from pigeons were collected from Jilin Province,northeastern China,between March and May 2015,and examined by nested PCR amplification of outer membrane protein A (ompA) gene.The overall Chlamydiosis prevalence was 5.01％ (21/399),with 3.19％ in Changchun City and 9.40％ in Jilin City.Furthermore,breed was the major risk factor associated with Chlamydia infection in pigeon,boiler pigeons had a prevalence of 7.49％,whereas no C.psittaci was detected in racing pigeons.Sequence analysis of the ompA gene revealed that all the identified isolates represented C.psittaci genotype B.Our results firstly indicated the presence of zoonotic C.psittaci in boiler pigeons in Jilin Province,northeastern China,and effective measures should be implemented to reduce the risk of C.psittaci transmission from pigeons to humans.