| 过氧化氢对PC12细胞plk1基因表达的影响 |
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| 引用本文： | 薛淑燕,吴心兰,伍秋婵,吴俐玫,陈东丽,梅寒芳.过氧化氢对PC12细胞plk1基因表达的影响[J].广东医药学院学报,2013(6):653-655. |
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| 作者姓名： | 薛淑燕 吴心兰 伍秋婵 吴俐玫 陈东丽 梅寒芳 |
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| 作者单位： | 广东药学院基础学院/广东省生物活性药物研究重点实验室,广东广州510006 |
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| 基金项目： | 广东省自然科学基金-博士启动项目（S2012040007968）;广东省中医药局建设中医药强省科研课题（20111246）;2012年广东省大学生创新训练项目（1057312027）;2012年广东药学院大学生创新实验项目 |
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| 摘 要： | 目的 观察过氧化氢对体外培养的PC12细胞plk1基因表达的影响.方法 采用MTT法观察不同浓度过氧化氢对体外培养的PC12细胞作用6h后,对PC12细胞存活率的影响;Western-blot检测不同浓度过氧化氢作用后,PC12细胞plk1基因蛋白表达水平的变化.结果 与对照组比较,过氧化氢剂量依赖性降低PC12细胞的存活率,增强PC12细胞plk1基因蛋白的表达水平.结论 过氧化氢降低PC12细胞的增殖活力,其机制可能是通过增强Plk1蛋白表达水平来实现.
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| 关 键 词： | 过氧化氢 PC12细胞 plk1基因 |
Effect of hydrogen peroxide on expression of plk1 gene in PC12 cells |
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| Authors: | XUE Shuyan WU Xinlan WU Qiuchan WU Limei CHEN Dongli MEI Hanfang |
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| Institution: | ( School of Basic Courses, Guangdong Pharmaceutical University / Guangdong Key Laboratory of Pharmaceutical Bioactive Substances, Guangzhou 510006, China) |
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| Abstract: | Objective To study the effect of hydrogen peroxide on the expression of plkl gene in PC12 cells cultured in vitro. Methods PC12 cells were incubated with different concentrations of hydrogen peroxide for 6 h,and then the cell viability was measured by MTT method, and the protein expression of polo-like kinase-1 （plkl） was detected by Western-blot method. Results Compared with the control group, hydrogen peroxide significantly decreased the cell viability of PC12 cells in the range of experimental dose. The protein expressional level of plkl gene in PC12 cells was also increased by hydrogen peroxide in a dose-dependent manner. Conclusion Hydrogen peroxide can directly inhibit the viability of PC12 cells ,which may be mediated by increasing the protein expressional level of plkl gene. |
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| Keywords: | hydrogen peroxide PC12 ceils polo-like kinase 1 gene（plkl gene） |
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