来氟米特联合贝那普利对糖尿病肾病大鼠ICAM-1表达的影响

目的 研究来氟米特联合贝那普利对糖尿病肾病大鼠对细胞间粘附分子（ICAM-1）表达的影响.方法 成年雄性Wistar大鼠,腹腔注射枸橼酸钠缓冲液,为正常对照组（NC组）,同批大鼠腹腔注射STZ（65 mg/kg）,建立糖尿病模型后,随机分为模型组（DN组）、贝那普利组、联合用药组.贝那普利组每天给予贝那普利灌胃（10 mg/kg/d）,联合用药组每天给予来氟米特和贝那普利灌胃（分别灌10 mg/kg/d）,NC和DN组每天给予等量蒸馏水灌胃.第4周、8周、12周末检测24小时尿蛋白排泄量（24UPE）和尿肌酐（Ucr）、血糖（Glu）、血肌酐（Scr）、计算内生肌酐清除率（Ccr）,12周末观察肾脏组织病理学变化及肾脏组织中ICAM-1的表达水平.结果 DN对照组ICAM-1在肾小球系膜区有强烈表达（P＜0.01）,贝那普利组较DN组表达减轻,联合用药组较贝那普利组表达更明显减轻（P＜0.05）.结论 来氟米特联合贝那普利可显著减少糖尿病肾病大鼠肾组织内ICAM-1表达,从而延缓糖尿病肾病的进展,其效果优于单用贝那普利.

Effect of combination of benazepril and leflunomide on ICAM-1 expression in the renal tissues of diabetic nephropathy rats

Objective To investigate the effect of combination of benazepril and leflunomide on expression of inter- cellular adhesion molecule （ICAM-1） in diabetic nephropathy （DN） model rats. Methods fifty for male Wistar rats were randomly divided into five groups., normal control （NC） group,DN group （DN）, benazepril group and combina- tion （benazepril ＋ leflunomide group. Diabetic Nephropathy models were induced by Intraperitoneal injection with Streptozotocin （STZ, 65 mg/kg） in the groups except NC. Benazepril group was intragastric administered with benaze- pril （10 mg/kg/d） and combination group was intragastric administered with leflunomide and benazepril （10 mg/kg/d, respectively）, Same volume of water was intragastric administered in the other groups. At the end of 4th, 8th and, 12th weeks,24-bour urinary protein excretion （24UPE） and urine creatinine （Ucr）,blood glucose level, serum creatinine （Scr） and endogenous creatinine clearance rate calculation （Ccr） were examined. At the end of 12th weeks,the patho- logical changes and expression of ICAM-1 were detected in the renal tissue. Results In DN group,ICAM-1 expression was significantly increased in mesangial region of glomerulus （P〈0.01 ,compared with NC group） ; the increases were attenuation in combination groups expression （P〈0.05, respectively）, and the＂ attenuation of combination group was larger than benazepril group（P〈0.05）. Conclusion Combination of leflunomide and benazepril can significantly re- duce the ICAM-1 expression in renal tissue in DN rats and can delay the progress of DN, and the effect is superior to benazepril alone.