Femtosecond laser-assisted selective reduction of neovascularization in rat cornea |
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Authors: | Mehra S Sidhu Min-Yeong Choi Suk-Yi Woo Hyun-Kyu Lee Heung-Soon Lee Kyu-Jin Kim Sae Chae Jeoung Jun-Sub Choi Choun-Ki Joo Il-Hong Park |
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Institution: | 1. Center for Medical Metrology, Division of Convergence Technology, Korea Research Institute of Standards and Science, Daejeon, 305 340, Republic of Korea 2. Department of Medical Physics, University of Science and Technology, Daejeon, 305 350, Republic of Korea 3. Department of Ophthalmology and Visual Sciences, Seoul St. Mary’s Hospital, The Catholic University of Korea and The Catholic Institute for Visual Science, Seoul, 137 701, Republic of Korea 4. HanbitNanoBioTech. Co., Ltd., Seoul, 152 848, Republic of Korea
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Abstract: | Nonlinear multiphoton absorption induced by focusing near infrared (NIR) femtosecond (fs) laser pulses into a transparent cornea allows surgery on neovascular structures with minimal collateral damage. In this report, we introduce an fs laser-based microsurgery for selective treatment of rat corneal neovascularizations (in vivo). Contiguous tissue effects are achieved by scanning a focused laser pulse below the corneal surface with a fluence range of 2.2–8.6 J/cm2. The minimal visible laser lesion (MVL) threshold determined over the corneal neovascular structures was found to be 4.3 J/cm2. Histological and optical coherence tomography examinations of the anterior segment after laser irradiations show localized degeneration of neovascular structures without any unexpected change in adjacent tissues. Furthermore, an approximately 30 % reduction in corneal neovascularizations was observed after 5 days of fs laser exposure. The femtosecond laser is thus a promising tool for minimally invasive intrastromal surgery with the aid of a significantly smaller and more deterministic photodisruptive energy threshold for the interaction between the fs laser pulse and corneal neovascular structures. |
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