固定抗凝剂量加入不同量脐血后脐血质量分析评估的研究

目的:研究固定抗凝剂量加入不同量脐血后对脐血质量的影响.方法:对6060份脐血样本,按照抗凝剂/脐血体积比分为28:(10-29)ml组、28:(30-69)ml组、28:(70-109)ml组、28:(110-150)ml组和28:(＞150)ml组.分析各组脐血的冻前有核细胞数、有核细胞活性、CFU-GM数量、CD...

The Impact of Certain Volume Anticoagulant on Cord Blood Quality in Different Volume of Cord Blood Units

Objective:To study the impact of anticoagulant to the quality of umbilical cord blood(UCB).Methods:6060 cord blood units(CBUs)were classified into five groups,such as 28 ml:(10-29)ml,28 ml:(30-69)ml,28 ml:(70-109)ml,28 ml:(110-150)ml and 28 ml:(>150)ml according to volume ratio of anticoagulant and CBVs.The count of pre-cryopreservation total nucleated cell(pre-TNC),the viability of nucleated cell(VNC),the amount of CFU-GM and the ratio changes of CD34+were evaluated and analyzed statistically.Results:It was found that pre-TNC increased with the growth of volume of CBUs(r=0.9937)under the certain volume of antico-agulant,and the TNC in the minimum UCB volume group was(2.57±0.89)×10^8;the VNC grew up with the increasing count viability of volume(r=0.9897),and the average viability of the minimum volume group remained over 95%;the CFU-GM climbed up with the increasing of volume(r=0.9024),and the number of CFV-GM in minimum volume group reached to of 89/×10^5;CD34^+%grew up with the increase of volume of CBUs(r=0.9641),and the ratio was(0.30±0.19)%for the minimum volume group.Conclusion:In certain volume of anticoagulant in collection-bag,pre-TNC,VNC,CFU-GM and CD34^+%are all dropped with the decrease of CBUs volume,however,all above-mentioned indexes in the minimun random group still meet the requirement for clinical administration.