白细胞来源微泡与急性髓系白血病微小残留病及预后的相关性

目的:探讨白细胞来源微泡与急性髓系白血病(AML)微小残留病(MRD)及预后的相关性。方法:用流式细胞仪检测47例AML患者诱导化疗后,21 d外周血的CD45⁺MP以及其中CD44⁺MP、CD24⁺MP的表达情况,并分析MP与MRD及预后的相关性。结果:MRD⁺组CD45⁺MP占比、CD44⁺MP占比、CD24⁺MP占比均显著高于MRD^-组;MRD⁺组CD45⁺MP、CD44⁺MP、CD24⁺MP与MRD水平呈正相关(r=0.589,r=0.548,r=0.711);CD45⁺MP、CD44⁺MP、CD24⁺MP预测MRD⁺的AUC分别为0.949、0.782、0.817;HCD45⁺MP组、HCD44⁺MP组、HCD24⁺MP组EFS及OS均显著短于低水平组(P<0.05)。结论:高水平CD45⁺MP、CD44⁺MP、CD24⁺MP可用来预测高水平MRD,高水平MP组预后差。

Relationship between Leukocytes Derived Microparticles and Minimal Residual Disease and Prognosis of Acute Myeloid Leukemia

Objective:To detect the relationship between leukocytes derived microparticle(CD45⁺MP)and minimal residual disease(MRD)and prognosis of acute myeloid leukemia(AML).Methods:The expression of CD45⁺MP,CD44⁺MP and CD24⁺MP in peripheral blood of 47 AML patients at the time after induction chemotherapy were detected by using flow cytometry,and the relationship between MP,MRD and prognosis were analyzed.Results:The percentages of CD45⁺MP,CD44⁺MP and CD24⁺MP in MRD positive group were significantly higher than those in MRD negative group.In MRD positive group,there were positive correlation between CD45⁺MP,CD44⁺MP,CD24⁺MP and MRD level.The AUC of CD45⁺MP,CD44⁺MP,CD24⁺MP in predicting positive MRD was 0.949,0.782,and0.817,respectively.The EFS and OS in HCD45⁺MP,HCD44⁺MP and HCD24⁺MP groups were significantly shorter than low level group.Conclusion:High level of CD45⁺MP,CD44⁺MP,CD24⁺MP can be used to predict high level MRD and poor prognosis.