雷公藤红素对人急性早幼粒白血病荷瘤模型小鼠黏附分子及细胞周期的影响

目的:观察雷公藤红素对人急性早幼粒白血病(APL)荷瘤模型小鼠黏附分子及细胞生物学特性的影响.方法:将18只SCID beige小鼠尾静脉注射NB4细胞株(5x106/只)以构建人APL荷瘤模型,然后随机分为荷瘤组、三氧化二砷组和雷公藤红素组,另取6只不造模并设为对照组;3周后向对照组和荷瘤组腹腔注射生理盐水并进行对照...

Effects of Tripterine on Adhesion Molecules and Cell Cycle in Human Acute Promyelocytic Leukemia Model Mice

Objective:To observe the effects of tripterine on adhesion molecules and cell biological characteristics in mice with acute promyelocytic leukemia(APL) tumor.Methods:Eighteen SCID beige mice were caudal vein injected with NB4 cell lines(5 × 106/only) to construct a human APL tumor-bearing model,then the mice were divided into tumor-bearing model group,arsenic trioxide group and tripterine group randomly,and another 6 mice which didn’t construct model were set up as control group;after 3 weeks,the control group and the tumor-bearing model were intraperitoneally injected with normal saline as compared,arsenic trioxide was intraperitoneally injected according to 100 μ g/kg,and tripterine was intraperitoneally injected according to 3 mg/kg.Four groups were all injected for 3 weeks.The biological characteristics of NB4 cells and the expression of adhesion molecules in venous blood of mice after treatment were observed.Results:The neutrophil decrased and promyelocytes,NB4 cells,B lymphocytes and white blood cells increased in tumor-bearing group as compared with control group(P<0.05),and the expressions of serum P-selectin(P-selectin),soluble vascular adhesion molecule-1(soluble vascular adhesion molecule-1,sVCAM-1) and soluble intercellular adhesion molecule-1(soluble intercellular adhesion molecule-1,sICAM-1) all increased(P<0.05).The cell cycle showed that the proportion of G0 and G1 increased,but S,G2-M phases obviously decreased(P<0.05).Compared with the tumor-bearing group,the neutrophils increased in the tripterine group,and the expression of early promyelocyte,NB4 cell,B lymphocyte,leukocyte,P-selectin,sVCAM-1 and sICAM-1 protein decreased(P<0.05),moreover,the ratio of G0 and G1 decreased,the G2-M and S phases increased(P<0.05).Among which sVCAM-1 and sICAM-1 of leucocyte,NB4 cell,P-selectin and adhesion molecules in tripterine group showed significant difference as compared with arsenic trioxide group(P<0.05).Conclusion:Tripterine may not only inhibit the expression of sVCAM-1 and sICAM-1 proteins in APL tumor-bearing mice and reduce the adhesion of tumor cells,but also block tumor cells at G2-M and S phases,thereby exerting therapeutic effect on APL.