TPX2在非肌层浸润性膀胱癌中的表达及其临床意义

目的： 研究Xklp2靶蛋白（TPX2）在非肌层浸润性膀胱癌中的表达及其临床意义。方法： 通过挖掘GEPIA数据库中的研究数据，对TPX2 mRNA在膀胱癌组织中的表达及其与患者生存的关系进行分析；采用免疫组织化学染色检测TPX2蛋白在60例非肌层浸润性膀胱癌石蜡标本组织中的表达，并分析其表达与肿瘤临床病理指标和患者预后的关系。结果： GEPIA数据库分析提示TPX2 mRNA在膀胱癌组织中的表达较正常组织升高（P<0.05），且TPX2 mRNA高表达组患者的无病生存率较低表达组患者降低（P<0.05）；免疫组织化学染色检测结果显示TPX2蛋白在非肌层浸润性膀胱癌组织中的阳性表达率为75%（45/60），而在对应的基底组织中，TPX2蛋白表达均为阴性，二者之间的差异具有统计学意义（P<0.01）；且TPX2的表达水平与患者肿瘤数目、肿瘤直径、病理分级及术后复发等病理指标有关（P<0.05），生存分析提示TPX2高表达的膀胱癌患者的生存期较低表达者短（P<0.05）。结论： TPX2在非肌层浸润性膀胱癌中表达上调，且其表达水平较高的患者预后较差，TPX2可能作为预测非肌层浸润性膀胱癌患者预后的肿瘤标记物。

Expression of TPX2 and its clinical significance in non-muscle-invasive bladder cancer

OBJECTIVE: To investigate expression of Xklp2 target protein (TPX2) in non-muscle-invasive bladder cancer and its clinical significance. METHODS: Data from the GEPIA database were analyzed to confirm expression of TPX2 mRNA in bladder cancer and its relationship with survival of patients. In addition,immunohistochemical staining was also used to detect the expression of TPX2 protein in 60 non-muscle-invasive bladder cancer tissues. Their relationships with clinical pathological indicator of tumors and prognosis of patients were analyzed. RESULTS: By mining the GEPIA database,TPX2 mRNA expressions were up-regulated in bladder cancer,and patients with higher expressions had lower disease-free survival rate (P<0.05). Immunohistochemical staining results showed that positive expression rate of TPX2 protein was 75% (45/60),while no positive expression was found in the corresponding basal tissues. The difference between the two groups was statistically significant (P<0.01). Expression levels of TPX2 protein were negatively correlated with clinical pathological indicators of tumors such as tumor numbers,tumor diameters,pathological grades and postoperative recurrences (P<0.05). Survival analyses showed that bladder cancer patients with higher expression of TPX2 had shorter survival time (P<0.05). CONCLUSION: Expression of TPX2 was up-regulated in bladder cancer and its expression level was negatively correlated with prognosis of patients. Therefore,it has potential to become a tumor marker for predicting prognosis in bladder cancer patients.