Tim-3在多发性骨髓瘤患者Th17/Treg细胞失衡中的意义

目的:探讨Tim-3在多发性骨髓瘤(MM)患者Th17/Treg失衡中的意义。方法:选取56例初诊MM患者及30例健康者,采用流式细胞术检测两组外周血CD4^+T细胞上Tim-3的表达、Th17和Treg细胞各占比例、Th17/Treg比值、Tim-3在Th17及Treg细胞上的表达、Tim-3^+Th17/Tim-3^+Treg比值。运用ELISA技术检测细胞因子IL-17和IL-10的水平。分析Tim-3^+Th17/Tim-3^+Treg平衡与临床指标的关系。结果:与对照组相比,MM患者外周血CD4^+T细胞上Tim-3表达明显增高(P<0.05)。MM患者组Th17细胞比例、Th17/Treg比值较对照组明显增高(P<0.05),MM患者Treg细胞比例较对照组低,差异无统计学意义(P>0.05)。MM患者组细胞因子IL-17、IL-10及IL-17/IL-10比值均较对照组明显增高(P<0.05)。MM患者组Tim-3^+Th17细胞水平及Tim-3^+Th17/Tim-3^+Treg比例较对照组明显增高(P<0.05),MM患者Tim-3^+Treg细胞水平较对照组低,差异有统计学意义(P<0.05)。MM患者Tim-3^+Th17/Tim-3^+Treg比值与ISS分期、DS分期、染色体异常及sFLCR呈正相关(r=0.635、r=0.501、r=0.449、r=0.587)。结论:MM患者体内存在Th17/Treg、IL-17/IL-10及Tim-3^+Th17/Tim-3^+Treg比值增高,其中Tim-3^+Th17/Tim-3^+Treg比值与患者ISS分期、DS分期、染色体异常及sFLCR有关,提示Tim-3参与了MM患者Th17/Treg失衡。

Significance of Tim-3 in The Imbalance of Th17/Treg in Patients with Multiple Myeloma

Objective:To investigate the singnificance of Tim-3 in Th17/Treg balance in patients with multiple myeloma(MM).Methods:Fifty-six newly diagnosed MM patients and 30 healthy people were enrolled.Flow cytometry was used to detect the expression of Tim-3 on CD4^+T cells,the proportion of Th17 and Treg cell,the Th17/Treg ratio,the expression of Tim-3 in Th17 and Treg cells,and the ratio of Tim-3^+Th17/Tim-3^+Treg cells in two groups.ELISA was used to detect the level of cytokines IL-17 and IL-10.Moreover,to analyze the relationship between Tim-3^+Th17/Tim-3^+Treg balance and clinical indicator.Results:Compared with the control group,the expression of Tim-3 on CD4^+T cells in peripheral blood of MM patients was increased(P<0.05),the ratio of Th17 cells and Th17/Treg in MM patients were higher than those in control group(P<0.05),and the ratio of Treg cells in MM patients was lower than those in control group,which the difference showed no statistically significant(P>0.05),and the level of cytokines IL-17,IL-10 and the ratio of IL-17/IL-10 in MM patients were significant higher than those in the control group(P<0.05).The expression level of Tim-3^+Th17 cells and the ratio of Tim-3^+Th17/Tim-3^+Treg in MM patients were higher than those in control group(P<0.05),while the expression level of Tim-3^+Treg cells in MM patients were lower than that in the control group(P<0.05).The ratio of Tim-3^+Th17/Tim-3^+Treg in MM patients were positive correlated with ISS staging,DS staging,chromosome abnormality and sFLCR(r=0.635,r=0.501,r=0.449,r=0.587).Conclusion:The ratios of Th17/Treg,IL-17/IL-10 and Tim-3+Th17/Tim-3+Treg increase in MM patients,among which Tim-3^+Th17/Tim-3^+Treg is correlated with ISS staging,DS staging,chromosomal abnormalities and sFLCR,which suggesting that Tim-3 is involved in the imbalance of Th17/Treg in MM patients.