ITP患者M-MDSC表达水平变化对其相关免疫功能的影响

目的:探讨单核细胞型髓系来源抑制性细胞(M-MDSC)在原发性免疫性血小板减少症(ITP)患者中表达水及Arg-1与iNOS的mRNA水平。分选CD4⁺T细胞、M-MDSC和CD14⁺HLA-DR⁺细胞。并以CFSE标记CD4⁺T细胞,ITP组患者外周血中Arg-1表达水平较对照组未见明显差异(P>0.05),而其iNOS表达水平明显升高(P<0.01)。ITP干扰素。结论:M-MDSC可能与ITP患者体内存在的免疫耐受功能紊乱有关,并可能成为监测ITP患者疗效的新指标。

Effect of Expression Level Changes of M-MDSC to Related Immune Function in Patients with Primary ITP

Objective:To investigate the effect of expression level changes of monocytic myeloid-derived suppressor cells(M-MDSC)to related immune function in the patients with primary immune thrombocytopenia(ITP).Methods:Peripheral blood samples were collected from 53 newly diagnosed ITP patients and 30 healthy volunteers.The quantity of M-MDSC,mRNA levels of Arg-1 and iNOS were detected.CD4⁺T,M-MDSC and CD14⁺HLA-DR⁺cells were sorted.CD4⁺T cells were marked by CFSE,and the immunosuppressive mechanism of M-MDSC was analyzed.Results:The count of M-MDSC in peripheral blood of newly diagnosed ITP patients was significantly higher than that in the control group(P<0.01).However,the expression level of Arg-1 in peripheral blood was not significantly different between the newly diagnosed ITP group and the control group.But the expression level of iNOS in the newly diagnosed ITP patients was significantly higher than that in the control group(P<0.01).After treatment,the count of M-MDSC in the patients with ITP was significantly lower than before treatment(P<0.01),which showed that M-MDSC could significantly inhibit the proliferation and secretion of EFN-γin CD4⁺T cells.Conclusion:M-MDSC may be related to the disorder of immune tolerance in the patients with ITP,and may become a new index to monitor the curative efficacy of ITP patients.