细胞内游离钙与柯萨奇病毒B_3诱导的大鼠培养心肌细胞凋亡(英文)

目的:探讨[Ca~(2 )]_i在柯萨奇病毒(CV)B_3诱导心肌培养细胞凋亡中的作用。方法:DNA裂点检测法(3'-末端标记)及扫描电镜检测细胞凋亡,Fluo3-AM负载心肌细胞,共聚焦显微镜观察[Ca~(2 )]_i荧光强度变化,结果:感染24 h,心肌细胞内CVB_3的浓度达峰值,感染10 h未见凋亡的心肌细胞,17,24和36 h凋亡细胞分别为5％,60％和90％,感染17 h心肌[Ca~(2 )]_i浓度达峰值,电镜结果提示凋亡的特征,结论:CVB_3可诱导培养心肌细胞的凋亡过程,细胞内Ca~(2 )参与凋亡细胞的信息转导过程,并在凋亡早期发挥重要作用。

Cytosolic-Ca~(2 ) and coxsackievirus B_3-induced apoptosis in cultured cardiomyocytes of rats

AIM: To explore the role of cytosolic free calcium ([Ca2 ]i) in apoptosis induced by coxsackievirus B3 ( CVB3 ) in cultured cardiomyocytes of rats. METHODS: Primary cultured cardiomyocyte was prepared from Wistar rats ages 2 - 3 d. The apoptosis in cardiomyocyte was determined by terminated deoxy-nucleotide transferase directed d-UTP nick and end labeling (TUNEL) method, and the apoptosis was observed under a transmission electron microscope. [Ca2 ]i in single cardiomyocyte loaded with Fluo 3-AM was measured by confocal microsorope. RESULTS: (1) The concentration of CVB3 in the medium reached the peak at 24 h after CVB3 infection. (2) The apoptotic cells were not found in CVB3-infected cardiomyocyte in first 10 h, but amounted to 5 % at 17 h, 60 % at 24 h, and 90 % at 36 h. (3) The peak value of [Ca2 ]i elevation reached at 17 h after CVB3 infection ( P < 0.01). (4) The characteristics of apoptosis was also seen by transmission electron microscope. CONCLUSION: CVB3 induced the apoptosis in cultured cardiomyocyte, and [Ca2 ]i mobilization was involved in the signal transduction process in apoptosis cells, and played an important role especially in the early stage of apoptosis induced by CVB3.