转染金属蛋白酶抑制剂对胃癌细胞体外侵袭能力的影响

目的：探讨基质金属蛋白酶抑制剂（ＴＩＭＰ－２）对ＳＧＣ－７９０１胃癌细胞体外侵袭能力的影响。方法：将人全长ＴＩＭＰ－２基因经脂质体包裹转染ＰＡ３１７细胞系，Ｇ４１８筛选，感染人ＳＧＣ－７９０１胃癌细胞系，经体外细胞增殖、细胞电泳率、软琼脂形成和侵袭小室的实验综合分析其生物学行为改变。结果：经ＴＩＭＰ－２基因转不的ＳＧＣ－７９０１细胞和ＭＦＣ细胞和细胞电泳率。软琼脂形成和侵袭能力均明显低于对照组，而

Effect of tissue inhibitor of matrix metalloproteinase-2 spontanous invasion of gastric carcinoma cell lines in vitro

Purpose:To study the effect on invasion behavior of gastric carcinoma in vitro of tissue inhibitior of matrix metalloproteinase 2 (TIMP 2) gene. Methods:A recombinant retroviral vector pl MT T2 which contained a flull length TIMP 2 complement DNA was transfered into PA317 cells by the liprofectmin technique. Human gastric cancer cell line SGC 7901 and mouse gastric cancer cell line MFC were used. Cell proliferation,sofa argon and boyden chamber were used to assay the change of invasion ability of transfered cell lines. Results:The electron rate,the ability of invasion of Boyden Chamber were significantly less than in the control groups. The curve ofcells transfered TIMP 2 gene were not significantly different from that of control group.Conclusions:TIMP 2 gene can change the quality of electrical charge on the surface of tumor cells and reverse the malignant phenotype of various type of tumor cells.