| 脑海绵状血管瘤遗传家系永生细胞株的建立 |
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| 引用本文： | 李敏,王立斌,王玉炯,马爱瑛,吕淑陶.脑海绵状血管瘤遗传家系永生细胞株的建立[J].第四军医大学学报,2007,28(2):140-142. |
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| 作者姓名： | 李敏 王立斌 王玉炯 马爱瑛 吕淑陶 |
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| 作者单位： | 宁夏大学生物技术重点实验室;宁夏大学生物技术重点实验室;宁夏医学院附属医院中心实验室,宁夏,银川,750021 |
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| 摘 要： | 目的:建立脑海绵状血管瘤家系永生细胞株.方法:利用EB病毒转化外周血B淋巴细胞,同时加入环孢霉素A的方法.结果:成功建立了该疾病家系7名成员永生细胞株,其中5名为患者.结论:脑海绵状血管瘤家系永生细胞株成功建立.
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| 关 键 词： | 血管瘤 海绵状中枢神经系统 疱疹病毒4型 人 B淋巴细胞 细胞系 |
| 文章编号： | 1000-2790(2007)02-0140-03 |
| 修稿时间： | 2006-06-23 |
Establishment of immortal lymphoblastoid cell lines in a pedigree of cerebral cavernous malformations |
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| LI Min,WANG Li-Bin,WANG Yu-Jiong,MA Ai-Ying,L Shu-Tao.Establishment of immortal lymphoblastoid cell lines in a pedigree of cerebral cavernous malformations[J].Journal of the Fourth Military Medical University,2007,28(2):140-142. |
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| Authors: | LI Min WANG Li-Bin WANG Yu-Jiong MA Ai-Ying L Shu-Tao |
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| Institution: | 1Key Labortory of Biotechnology, Ningxia University;2 Central Laboratory, Affiliated Hospital, Ningxia Medical College, Yinchuan 750021, China |
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| Abstract: | AIM: To establish immortal lymphoblastoid cell lines in a pedigree of cerebral cavernous malformations(CCM). METHODS: The cell lines were constructed by means of adding Epstein-Barr virus and Cyclosporine A onto peripheral B lymphocytes. RESULTS: We successfully established 7 immortal lymphoblastoid cell lines in the family, and 5 of them suffered from CCM. CONCLUSION: These immortal lymphoblastoid cell lines would preserve the unique genome of CCM. |
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| Keywords: | hemangioma cavernous central nevvous system herpesvirus 4 human B lymphocytes Cell line |
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