老年大鼠脑出血后神经细胞再生的研究

目的：探讨老年大鼠脑出血后发生的神经细胞再生现象及其特点。方法：应用成年及老年（分别3个月及18个月大小）雄性Sprague—Dawley大鼠制作脑出血模型，主要采用蛋白印迹分析、免疫组织化学染色等方法，对Bromodeoxyuridine（Brdu）及未成熟神经元标记doublecortin（DCX）和polysialylated neural cell adhesion protein（PSA—NCAM）等进行分析。结杲：在老年大鼠，DCX的表达于脑出血后7天即有增加，2周后同侧脑室下区（subventricular zone，SVZ）及基底节可见大量DCX阳性细胞，其表达达到高峰，是对照组的（670±145）％（P〈0．01）；绝大多数的DCX阳性的细胞也共同表达Brdu；与成年大鼠相比，老年大鼠脑出血后DCX表达减少。结论：老年大鼠脑出血后有明显的神经细胞再生，SVZ的神经元前体细胞增生明显，并向受损伤的基底节区移行。

Neuron regeneration in aged rats after intracerebral hemorrhage

OBJECTIVE: To investigate neuron regeneration of aged rats after intracerebral hemorrhage(ICH). METHODS: ICH model was induced by intracerebral infusion of 100muL autologous blood in adult and aged (3 and 18 m of age, respectively) male Sprague-Dawley (SD) rats. Doublecortin(DCX), polysialylated neural cell adhesion protein(PSA-NCAM) and Bromodeoxyuridine (Brdu) in brain tissue were detected by Western blot analysis and immunohistochemical examination. RESULT: In aged rats, DCX levels in the ipsilateral basal ganglia and subventricular zone started to increase at 7d after ICH, peaked at 14 d [(670+/-145)% of controls, P<0.01]. Most DCX positive cells in ipsilateral striatum were BrdU positive. Though DCX content in ipsilateral basal ganglia [(2560+/-758) pixels]of ICH aged rats was much higher than that of aged rat controls [(450+/-97)pixels, P<0.01], it was still lower than that in adult rats. Conclusion: The results demonstrate that neuron regeneration occurs in subventricular zone in the aged rats following intracerebral hemorrhage(ICH).