中药微量元素Cu和总黄酮测定与清除自由基功效关系

目的:通过对治疗呼吸系统疾病中草药中微量元素铜和总黄酮的含量测定,分析微量元素Cu与总黄酮含量的相关性。方法:选取8种中草药板蓝根、五味子、黄芩、紫草、银杏、金银花、柴胡、川芎,粉碎、干燥至恒重,用原子吸收分光光度计测定其微量元素铜的含量,采用70%醇提法,提取药物中总黄酮,用紫外分光光度法测定药物中总黄酮的含量。分析研究黄酮化合物与微量元素Cu协同抗氧化清除自由基的作用机制及其功效。结果:8种治疗呼吸系统疾病中草药中含有丰富的微量元素Cu,含量最高的是柴胡,含量最低的是板蓝根,总黄酮含量较高的有银杏叶、黄芩、川芎、紫草、金银花。结论:不同药物中所含的总黄酮和Cu元素含量具有一定的差异,药材生长环境不同,产地不同,会影响到化学成分的含量,从而影响到药效。银杏叶、金银花、紫草中Cu的含量较高,这几种药物中总黄酮的含量也较高。中药中的黄酮类化合物和微量元素均具有重要的作用,但并不是单独发挥其药效作用,微量元素与黄酮可结合成金属配合物,测定结果为进一步探讨草药抗衰老、清除自由基的机理提供依据。

Determination on the Contents of Cu, Flavone in Chinese Herbal Medicines and Analysis the Relation of the Effect for Eliminating free Radical

Objective:This experiment determinated the content of Mn and flavonoid in Chinese Herbal medicines,and analyzed trace element Cu with the relativity of the total flavonoid content,and the effect of the clearance free radicals relation.Method:The medicines were Radices jsatidis seu baphicacanthi,Sehizandra sinensis Bail,Scutellaria baicalensis Georgi,Lithospermumofficinalel,Lonicera japonica,Bupleurum falcatuml,Ligusticum wallichii,dried and powdered,completely mixed,The atomic absorption spectrometry was used to determine the contents of trace elements Cu,Adopt alcohol extract the flavone in Chinese Herbal medicines,and the content of flavone was determinted with uv spectrometry.Results:The results shows that metal elements Cu and flavone were rich in 8 kinds of Chinese Herbal medicines.There was the most trace element Cu in Bupleurum falcatuml,and the lowest trace element Cu in Radices jsatidis seu baphicacanthi.There were more flavone in Salisburia adiantifolia,Scutellaria baicalensis Georgi,Ligusticum wallichii,Lithospermum officinalel,Lonicera japonica.Conclusion: In the different medicine content with of total flavonoid and trace element Cu contents have certain difference,the medicine material growth environment dissimilarity,the place dissimilarity,meeting influence the chemistry composition,thus affect the efficacy of medicine.The content of the chemical element of Cu is higher in the gingko leaf,honey-suckle,purple grass,the content of the total flavonoid is also higher in this a few medicine.Flavonoid compound in the Chinese herbal medicine and trace element all the one who have its important function,but is not alone exertive its efficacy of medicine function,trace element and flavonoids can combine metals matches with the thing,can develop the anti-oxidizes the function,the clearance free radicals inside the body.It provided a useful data for the further study mechanism of the herb medicine anti-decrepitude,the clearance free radicals.