| 环磷酰胺诱发的染色体损伤与P53基因表达的关系 |
| |
| 引用本文： | 王明艳,许冬青,徐力,张旭,赵凤鸣,姜海英. 环磷酰胺诱发的染色体损伤与P53基因表达的关系[J]. 癌变．畸变．突变, 2003, 15(4): 228-230. DOI: 10.3969/j.issn.1004-616x.2003.04.013 |
| |
| 作者姓名： | 王明艳 许冬青 徐力 张旭 赵凤鸣 姜海英 |
| |
| 作者单位： | 南京中医药大学基础医学院生物教研室,江苏,南京,210029;南京中医药大学学报编辑部,江苏,南京,210029 |
| |
| 基金项目： | 江苏省教育厅自然科学基金;KJB360006; |
| |
| 摘 要： | 目的：探讨环磷酰胺(cyclophosphamide，CP)对染色体损伤与P53基因表达间的关系。 方法：以正常人外周血淋巴细胞为材料，用流式细胞仪检测环磷酰胺对突变型P53基因表达的影响，同时以微核(MN)及姐妹染色单体互换(SCE)为指标，进行致突变研究。 结果：环磷酰胺用药组突变型P53基因表达率为30.81 ％，微核率为8 ‰，姐妹染色单体互换率为9.21±1.08，与对照组比较均有显著差异。 结论：环磷酰胺的致突变作用可能与影响P53的基因的表达有关。
|
| 关 键 词： | 环磷酰胺 致突变 突变型P53基因 微核 姐妹染色单体互换 |
| 文章编号： | 1004-616X(2003)04-0228-03 |
| 收稿时间： | 2002-07-17 |
| 修稿时间： | 2002-07-17 |
RELATIONSHIP BETWEEN CHROMOSOME DAMAGE INDUCED BY CYCLOPHOSPHAMIDE AND EXPRESSION OF MUTATED P53 GENE |
| |
| WANG Ming-yan,XU Dong-qing,XU Li,et al. RELATIONSHIP BETWEEN CHROMOSOME DAMAGE INDUCED BY CYCLOPHOSPHAMIDE AND EXPRESSION OF MUTATED P53 GENE[J]. Carcinogenesis,Teratogenesis and Mutagenesis, 2003, 15(4): 228-230. DOI: 10.3969/j.issn.1004-616x.2003.04.013 |
| |
| Authors: | WANG Ming-yan XU Dong-qing XU Li et al |
| |
| Affiliation: | 1.Biological Teaching and Researching Section, Basic Medical College of Nanjing University of Traditional Chinese Medicine,2.The Editorial Department of Journal of Nanjing University of TCM, Nanjing 210029,China |
| |
| Abstract: | Purpose: To study the relation between chromosome damage induced by cyclophosphamide(CP) and the expression of mutated P53 gene. Methods: The expression of mutated P53 gene in human lymphocytes was investigated using flow cytometry.And the micronucleus(MN) and sister chromatid exchange (SCE) tests were studied in vitro. Results: The expression ofmutated P53 gene in human lymphocytes treated with CP was 30.81 ％,(P < 0.01)compared with the control .The frequencies of SCE and MN in human lymphocytes exposed with CP were 9.21±1.08 and 8 ‰ respectively(P < 0.01),compared with the control. Conclusion: The mutagenic effect of CP may be related to the expression of mutated P53 gene . |
| |
| Keywords: | cyclophosphamide mutagenicity mutated P53 gene micronucleus sister chromatid exchange |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《癌变．畸变．突变》浏览原始摘要信息 |
|
点击此处可从《癌变．畸变．突变》下载全文 |
