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| 中国脑血栓患者家系凝血因子Ⅸ基因SNP9的检测 | |
| 引用本文： | 闫伟,孟晓萍,杨丽丽,康平,Tom Howard.中国脑血栓患者家系凝血因子Ⅸ基因SNP9的检测[J].中国组织工程研究与临床康复,2003,7(16):2257-2258. |
| 作者姓名： | 闫伟 孟晓萍 杨丽丽 康平 Tom Howard |
| 作者单位： | 1. 吉林大学第二医院,神经内科,吉林省长春市,130041 2. 吉林大学第二医院,心血管内科,吉林省长春市,130041 3. 吉林省电力医院内科,吉林省长春市,130021 4. 美国Emory大学 |
| 摘 要： | 目的凝血因子Ⅸ基因在第六外显子
23384～ 23387 bp存在单核苷酸多态性 (SNP9).探讨这一单核苷酸的多态性在中国北方人群脑血栓发病机制中的作用.方法应用聚合酶链反应-限制性片断长度多态性
(PCR- RFLP)的方法,检测 20个脑血栓患者和他们家系, 60例正常健康人群凝血因子Ⅸ第六外显子
2338～ 23387 bp多态性分布.结果 20例脑血栓患者和 40例他们的家系成员,在
MnⅡ酶切后,可见 307 bp片断基因类型全部是 A.对照组 60例基因多态性基因型也显示为
A,阳性对照可见 A、 G和 AG.结论在这一等位基因位点脑血栓家系和正常人群基因多态性基因型均为
A型. |
| 关 键 词： | 多态性 单核苷酸 颅内血栓形成 因子 IX 等位基因 |
Measure coagulation factor Ⅸ 's gene SNP9 of cerebral thrombosis patients' genealogy in China |
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| Tom Howard.Measure coagulation factor Ⅸ ''s gene SNP9 of cerebral thrombosis patients'' genealogy in China[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2003,7(16):2257-2258. | |
| Authors: | Tom Howard |
| Abstract: | Aim Coagulation factor-Ⅸ gene appears SNP9 in exon 6 between 23 384- 23 387 bp in cerebral thrombosis patients and exploring effect of SNP9 on pathogeny of patients in northeastern China.Methods PCR- restriction fragment length polymorphism(PCR- RFLP) was adopted in genotyping SNP9 in 20 patients and their genealogy, as well as 60 random healthy individuals as control.Results SNP9 genotype of 307 bp fragment was all A type after dissolved by MnII enzyme in 20 patients with cerebral thrombosis,their genealogy and 60 healthy people.type A,type G and type AG were found in positives contrastive people.Conclusion Genotype of genetic polymorphism are type A in alleles of cerebral thrombosis patients and those of control. |
| Keywords: | polymorphism single nucleotide intracranial thrombosis factor IX alleles |
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