人参皂苷Rb1对癫痫大鼠海马组织线粒体的保护作用和机制探讨

目的探讨人参皂苷Rb1对戌四氮(PTZ)致痫大鼠海马线粒体结构和功能的作用及机制。方法采用PTZ 40mg·kg~(-1)·d~(-1)腹腔注射制备癫痫动物模型,绐予人参皂苷Rb1低剂量20mg·kg~(-1)·d~(-1)和高剂量80mg·kg~(-1)·d~(-1)连续干预30d后,分别检测海马组织中细胞内活性氧(ROS)和线粒体膜电位(△Ψm)的水平,MDA和SOD的含量,及胞质中Cytochrome c和Caspase3的表达,并观察海马CA1区线粒体的超微结构。结果与对照组相比,模型组大鼠海马中ROS水平升高,△Ψm水平降低(P0.01);MDA含量升高,SOD活性减低(P0.01),胞质中Cytochrome c和Caspase3的表达亦相应增加(P0.05),且线粒体结构明显空泡化;而人参皂苷Rb1干预组剂量依赖性逆转了上述指标的异常改变。结论人参皂苷Rb1可挽救致痫大鼠海马组织中线粒体继发损伤,其机制可能与抗氧化、抗凋亡有关。

Effects of ginsenoside Rb1 on mitochondrial damages of hippocampus in epileptic rats and related mechanism

Objective To explore the effects of ginsenoside Rb1 on mitochondrial damages of hippocampus in pentylenetetrazol ( PTZ)-induced epileptic rats and related mechanism .Methods Rats were given PTZ 40mg? kg-1? d-1 and ginsenoside Rb1 20mg? kg-1 ? d-1 and 80mg? kg-1 ? d-1 .After 30 days, levels of reactive oxygen species ( ROS) and mitochondrial membrane potential ( ΔΨm), and levels of MDA and SOD were determined, respectively.Meanwhile, expressions of Cytochrome c and Caspase 3 in the hippocampus cytoplasm were detected.What's more, mitochondrial ultrastructures were observed by electron microscope .Rseults Compared to the control group, increased ROS and decreased ΔΨm (P<0.01),and increased MDA and decreased SOD (P<0.01) were indicated in the PTZ group .Meanwhile, increased expressions of Cytochrome c and Caspase 3 (P<0.05) and obvious mitochondria vacuolations were also demonstrated in the PTZ group , while ginsenoside Rb1 could dose-dependently reversed these changes.Conclusion Ginsenoside Rb1 could effectively prevent hippocampal mitochondria damages of epileptic rats , which might be relevant to its anti -oxidative stress and anti-apoptosis properties.