| c-myc反义寡核苷酸对HL-60细胞增殖及c-myc基因表达的影响 |
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| 引用本文： | 孙玲,王峰,乐晓萍,孙慧,姜中兴,程远东,陈绍倩,王一浩,马鸿雁,张钦宪. c-myc反义寡核苷酸对HL-60细胞增殖及c-myc基因表达的影响[J]. 郑州大学学报(医学版), 2005, 40(2): 278-281 |
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| 作者姓名： | 孙玲 王峰 乐晓萍 孙慧 姜中兴 程远东 陈绍倩 王一浩 马鸿雁 张钦宪 |
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| 作者单位： | 郑州大学第一附属医院血液内科,郑州,450052;郑州大学医学院分子细胞生物学研究中心,郑州,450052 |
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| 基金项目： | 河南省医学创新人才基金资助项目 2004107031 |
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| 摘 要： | 目的：观察c-myc基因反义寡核苷酸(ASODN)对HL-60细胞增殖及c-myc基因表达的抑制作用．探讨ASODN作为药物进行白血病基因治疗的可行性、方法：应用c-myc反义寡核苷酸封闭HL-60细胞c-myc基因、RTPCR方法检测该基因表达抑制情况．流式细胞仪进行细胞周期分析，倒置显微镜观察细胞形态变化．绘制细胞生长曲线，MTT法检测细胞增殖状态。结果：c-myc基因反义寡核苷酸转染HL-60细胞后，c-myc基因表达明显受抑；S期细胞比值由55.6％降至16．7％；细胞生长受抑，增殖能力减弱，其抑制作用具有序列特异性及剂量依赖性。结论c-myc基因反义寡核苷酸对HKL-60细胞增殖及c-myc基因的表达具有明显的抑制作用，有望成为抑制白血病细胞增殖的核酸药物。
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| 关 键 词： | HL-60细胞 c-myc基因 反义寡核苷酸 细胞周期 |
| 修稿时间： | 2004-08-25 |
Inhibition effect of c-myc antisense oligodeoxynucleotide on the proliferation and expression of c-myc gene in HL-60 cells |
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| SUN Ling,WANG Feng,LE Xiaoping,SUN Hui,JIANG Zhongxing,CHENG Yuandong,CHEN Shaoqian,WANG Yihao,MA Hongyan,ZHANG Qinxian. Inhibition effect of c-myc antisense oligodeoxynucleotide on the proliferation and expression of c-myc gene in HL-60 cells[J]. Journal of Zhengzhou University: Med Sci, 2005, 40(2): 278-281 |
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| Authors: | SUN Ling WANG Feng LE Xiaoping SUN Hui JIANG Zhongxing CHENG Yuandong CHEN Shaoqian WANG Yihao MA Hongyan ZHANG Qinxian |
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| Affiliation: | SUN Ling 1),WANG Feng 2),LE Xiaoping 2),SUN Hui 1),JIANG Zhongxing 1),CHENG Yuandong 1),CHEN Shaoqian 1),WANG Yihao 1),MA Hongyan 1),ZHANG Qinxian 2) 1)Department of Hematology,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052 2)Molecular Cell Biology Research Center,Medical College,Zhengzhou University,Zhengzhou 450052 |
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| Abstract: | Aim: To study the inhibition effect of c-myc antisense oligodeoxynucleotide(ASODN) on the expression of c-myc gene and the proliferation of HL-60 cells and to explore the possibility of ASODN as a drug for leukaemia gene therapy.Methods: After treated by c-myc ASODN,the expression of c-myc mRNA was detected by RT-PCR in HL-60 cells. The cell cycle was analyzed by flow cytometric analysis. The morphological changes were observed under invert microscope.The cell proliferation was investigated by growth curve and MTT method. Results: After sealing c-myc gene with ASODN,the expression of c-myc gene was inhibited. HL-60 cells in S phase decreased from 55.6% to 16.7%.The growth of cells was repressed and the ability of proliferation decreased.The c-myc ASODN acted as a specific growth inhibitor to HL-60 cell and the effect was dose-dependent and specific in sequence.Conclusion: The c-myc ASODN can inhibit the proliferation of HL-60 cells by inhibiting the expression of c-myc gene and may be a new gene drug to inhibit the proliferation of leukaemia cells. |
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| Keywords: | HL-60 cells gene c-myc oligodeoxynucleotide antisense cell cycle |
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