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Differences of Accumulation of the Major Secondary Metabolites in Paris L. Leaves

??OBJECTIVE To investigate the differences of the accumulation of the main secondary metabolites in the leaves of 11 kinds of Paris L. medicinal plants. METHODS The contents of the main secondary metabolites, polyphyllin??, ??, ??, and ?? in the leaves of 11 kinds of Paris L. medicinal plants were determined by UPLC, and the UPLC fingerprints were established. The accumulation of the main secondary metabolites was evaluated by one-way ANOVA and chromatographic analysis. RESULTS There was significant difference(P<0.01)in the contents of polyphyllin ??, ??, ??, and ?? in the leaves of 11 kinds of Paris L. medicinal plants, and polyphyllin ??, ??, ??, ?? were simutaneously detected only in var. yunnanensis-1, P. axialis, P. thibetica, P. forrestii and var. yunnanensis; there was significant difference in the UPLC chromatograms of 11 kinds of Paris L. medicinal plants, but the similarities among var. yunnanensis-1, P. axialis, P. thibetica, P. forrestii and var. yunnanensis all reached 0.902 with 16 common peaks, indicating smaller difference in their main secondary metabolites. CONCLUSION There is significant difference in the abilities of the 11 kinds of Paris L.medicinal plants to accumulate polyphyllin ??, ??, ??, and ?? , which may be the main reason that there are significant differences in the contents and classes of the major secondary metabolites of Paris L. roots.