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| 6-O-��炙��Ǿ������γ��������绯ø���Ʊ��������о� | |
| 引用本文： | ���Ừ,�δ�ϼ,����ɽ,�����.6-O-��炙��Ǿ������γ��������绯ø���Ʊ��������о�[J].中国药学杂志,2015,50(10):834-839. |
| 作者姓名： | ���Ừ �δ�ϼ ����ɽ ����� |
| 作者单位： | 1.???????????????????????о?????,???? 250012; 2.????????????У??????,??? ???? 264209 |
| 摘 要： | |
| 关 键 词： | ?????????绯? 6-O-??炙?????? ??????? ???? |
Preparation and Characterization of Superoxide Dismutase Chemically Modified by 6-O-Quaternized Chitosan |
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| XU Ling-hua,SONG Chun-xia,WANG Feng-shan,LIU Chun-hui.Preparation and Characterization of Superoxide Dismutase Chemically Modified by 6-O-Quaternized Chitosan[J].Chinese Pharmaceutical Journal,2015,50(10):834-839. | |
| Authors: | XU Ling-hua SONG Chun-xia WANG Feng-shan LIU Chun-hui |
| Institution: | 1.School of Pharmaceutical Sciences &National Glycoengineering Research Center, Shandong University, Jinan 250012, China; 2.School of Ocean Biology, Shandong University at Weihai, Weihai 264209, China |
| Abstract: | ??OBJECTIVE To chemically modify superoxide dismutase (SOD) with 6-O-quaternized chitosan. METHODS 2,3-Epoxypropyl trimethyl ammonium chloride was grafted to reactive C6-OH of chitosan to obtain O-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (O-HTCC). O-HTCC was covalently coupled to SOD via EDC/NHS-mediated reaction,followed by purification on DEAE Sepharose fast flow column. The conjugates were analyzed by SDS-PAGE, HPLC,and circular dichroism. The enzyme activity of the conjugates was determined using pyrogallol autoxidation assay. RESULTS SOD was successfully modified by O-HTCC at an efficiency of 90%. The homogeneous conjugates showed similar secondary structure and retained 81.1% of enzyme activity compared with native SOD.CONCLUSION SOD can be efficiently modified by O-HTCC to prepare O-HTCC-SOD conjugates with high activity retention rate. |
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