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Influence of TPGS 1000 and Soluplus on Membrane Transport of Ginsenoside CK in Caco-2 cell Monolayer Model

??OBJECTIVE To examine the effects of TPGS 1000 and Soluplus on the transport of ginsenoside CK in Caco-2 cell model. METHODS The effects of TPGS 1000 and Soluplus at different concentrations on ginsenoside CK were evaluated by using Caco-2 cell model. The concentration of ginsenoside CK in cell was examined by ultra high pressure liquid chromatography (UPLC) method. The apparent permeability coefficient (P_app) and the efflux ratio were calculated. RESULTS When the proportion of ginsenoside CK to TPGS 1000 or Soluplus was 1??1, 1??3, and 1??9, the absorption of ginsenoside CK significantly increased. The efflux and efflux ratio both decreased significantly(P<0.05). TPGS 1000 had more significant promotion effect on the transport of ginsenoside CK than the same dose of soluplus at the same ratio (P<0.05). CONCLUSION In Caco-2 cell model, both TPGS 1000 and Soluplus can significantly promote the absorption of ginsenoside CK.