ITS2???м??????????????? Identification of <i>Ilex asprella</i> and Its Closely Related Species Based on ITS2 Barcode期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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引用本文：	???,????,?γ?,?±???,??????,???.ITS2???м???????????????[J].中国药学杂志,2015,50(17):1505-1511.

作者姓名：	??? ???? ?γ? ?±??? ?????? ???

作者单位：	1. ?й????????????о???, ????????????????????????????????, ???? 100700; 2. ??????????????, ?人 430065; 3. ???????????о??, ???? 530001; 4. ????????????????о??, ?人 430064

摘要：
关键词：	????? ITS2???? ???? ?????α?
*Identification of Ilex asprella* and Its Closely Related Species Based on ITS2 Barcode**

ZHOU Hong,MA Shuang-jiao,SONG Chi,CHEN Bei-bei,HAN Zheng-zhou,YAO Hui.Identification of Ilex asprella and Its Closely Related Species Based on ITS2 Barcode[J].Chinese Pharmaceutical Journal,2015,50(17):1505-1511.

Authors:	ZHOU Hong MA Shuang-jiao SONG Chi CHEN Bei-bei HAN Zheng-zhou YAO Hui

Institution:	1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Science, Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Beijing 100700, China; 2. College of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430065, China; 3. Guangxi Institude of Minority Medicine, Nanning 530001, China; 4. Hubei Institute for Food and Drug Control, Wuhan 430064, China

Abstract:	??OBJECTIVE To identify 93 samples of Ilex asprella(Hook. et Arn.) Champ. ex Benth. and its related species based on ITS2 barcode. METHODS The total genomic DNAs were extracted and the ITS2 regions were amplified and sequenced. The genetic distances were computed using MEGA5.0 in accordance with the Kimura 2-parameter (K2P) model. The neighbor-joining (NJ) phylogenetic tree and BLAST method were used to assess the efficiency of sequence identification. RESULTS The length of ITS2 sequence of I. asprella was 241 bp and the average GC content was 63.4%. The intra-specific genetic distances of I. asprella were 0-0.030 and the minimum inter-specific of I. asprella and its closely related species was 0.043. Furthermore, I.asprella formed into a single branch with high bootstrap value in NJ tree. The identification efficiency of ITS2 barcode using BLAST was 100%. CONCLUSION The ITS2 region can accurately and stably distinguish I.asprella from its closely related species and adulterants.It provides a new method to ensure the clinical safety of traditional Chinese medicines.

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