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Simultaneous Determination of Eight Water-Soluble Constituents in Zhejiang Fritillary Slices Before and After Sulfur Fumigation Process by HPLC-DAD

??OBJECTIVE To establish a method for simultaneous determination of adenine, cytidine, inosine, uracil, uridine, thymidine, adenosine and 2-deoxyadenosine in Zhejiang Fritillary Slices, and determine and compare the contents of the eight compositions in Zhejiang Fritillary Slices before and after sulfur fumigation process. METHODS The samples solution was determined by HPLC-DAD with an Inertsil ODS-SP C₁₈ column (4.6 mm??250 mm,5.0 ??m) using water (A)-methanol (B) as the mobile phase by gradient elution (0-10 min,1%??5%B;10-15 min,5%??15%B;15-20 min,15%??20%B;20-30 min,20%B;30-35 min,20%??100%B) at the flow rate of 1 mL??min^-1. The column temperature was maintained at 25 ??. RESULTS All the components showed good linearity (r??0.999 5) in the range of the tested concentration. The average recoveries of the method were 100.90%, 97.92%, 100.28%, 98.95%, 100.42%, 99.02%, 101.96% and 100.39%, respectively. After sulfur-fumigation process, the contents of the six compositions in Zhejiang Fritillary Slices decreased obviously. CONCLUSION The validated method has the advantages of simpleness, high precision and reliability, allowing the comprehensive quality control of Zhejiang Fritillary Slices. Sulfur fumigation can remarkably affect the amount of the eight water-soluble constituents in Zhejiang Fritillary Slices. So, sulfur fumigation shouldn??t be apllied as primary processing method for Zhejiang Fritillary Slices.