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Sustained-Release Effect of Na-Montmorillonite on 1-Deoxynojirimycin in Alkaloid Fraction of Mulberry Leaf Extract

??OBJECTIVE To investigate the sustained-release ability of Na-montmorillonite (Na-MMT) on 1-deoxynojirimycin (DNJ) in mulberry leaf extract (MLE). METHODS In order to prepare DNJ-MMT composite, DNJ was intercalated into the interlayer of MMT by stirring. Time and temperature for adsorption and ratio of DNJ to MMT were determined by calculating the loading of DNJ, then in vitro release experiment was carried out to estimate the sustained-release characteristics of DNJ-MMT. RESULTS DNJ-MMT composite with a drug loading of 111.64 mg??g^-1 was obtained by stirring 5.00 g Na-MMT and 16.00 g MLE containing 4 016.0 mg DNJ in 1 000 mL deionized water at 100 ?? for 1 h. The composite released DNJ quickly in water, potassium phosphate buffer (pH 7.4), and 0.1 mol??L^-1 hydrochloric acid solution. With the increasing of volume of hydrochloric acid solution, the release ratio of DNJ also increased correspondingly. CONCLUSION The sustained-release effect of Na-MMT on DNJ is proved. Na-MMT can be used for study of DNJ sustained-release preparations.