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| ????ITS2???м?????????????????α? | |
| 引用本文： | ?????,???,?ξ??.????ITS2???м?????????????????α?[J].中国药学杂志,2015,50(15):1286-1291. |
| 作者姓名： | ????? ??? ?ξ?? |
| 作者单位： | 1. ?й???????????Э????????????о???,??Σ??????????????????,????100193; 2. ?????????????о???,????408435 |
| 摘 要： | |
| 关 键 词： | ???? DNA?????? ??????? ?г???? |
Molecular Identification of Dalbergiae Odoriferae Lignum and Its Adulterants Using the ITS2 Sequence |
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| HOU Dian-yun,ZHOU Hong,SONG Jing-yuan.Molecular Identification of Dalbergiae Odoriferae Lignum and Its Adulterants Using the ITS2 Sequence[J].Chinese Pharmaceutical Journal,2015,50(15):1286-1291. | |
| Authors: | HOU Dian-yun ZHOU Hong SONG Jing-yuan |
| Institution: | 1. The National Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China; 2. Chongqing Institute of Medicinal Plant Cultivation, Chongqing 408435, China |
| Abstract: | ??OBJECTIVE To identify Dalbergiae Odoriferae Lignum and its adulterants using DNA barcoding. METHODS ITS2 is one of the popular DNA barcoding in the identification of traditional Chinese medicine. In this paper,the ITS2 regions of Dalbergiae Odoriferae Lignum and its adulterants were amplified and sequenced bi-directional.The length and GC content of ITS2 sequence were analyzed through MEGA5.0 software. The genetic distances were computed by kimura 2-parameter (K2P) model. Dalbergiae Odoriferae Lignum and its adulterants have been identified through the species identification system for traditional Chinese medicine and neighbor-joining (NJ) phylogenetic tree. RESULTS The sequence lengths of ITS2 of Dalbergiae Odoriferae Lignum were 216 bp, and the GC content was 68.5%. The minimum K2P interspecific genetic distances of Dalbergiae Odoriferae Lignum and its adulterants were 0.009, which was larger than that of the intraspecific genetic distances of Dalbergiae Odoriferae Lignum.The Dalbergiae Odoriferae Lignum and its adulterants can be obviously identified using the Species identification System and NJ phylogenetic trees.CONCLUSION ITS2 Regions as DNA barcode can identify Dalbergiae Odoriferae Lignum and its adulterants accurately. |
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