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Simultaneous Determination of Five Effective Components in Different Parts of Dracocephalum moldevica L by RP-HPLC

??OBJECTIVE To develop an RP-HPLC method for simultaneous determination of rosmarinic acid, tilianin, luteolin-7-O-??-D-glucuronide, apigenin-7-O-??-D-glucuronide, and diosmetin-7-O-??-D-glucuronide in different parts of Dracocephalum moldevica L. METHODS The five constituents were measured on a Shim-pack ODS column(4.6 mm??250 mm, 5 ??m)with gradient elution of acetonitrile (A)-0.5% formic acid aqeous solution (B) (0-30 min, 17%A;30-60 min, 17%-28%A; 60-70 min, 28%A) at a flow rate of 1.0 mL??min^-1.The detection wavelength was set at 330 nm, and the column temperature was maitained at 35 ??. RESULTS The linear ranges of rosmarinic acid, tilianin, luteolin-7-O-??-D-glucuronide, apigenin-7-O-??-D-glucuronide, and diosmetin-7-O-??-D-glucuronide were 4.2-126 ??g??mL^-1 (r=0.999 2), 7.84-235.2 ??g??mL^-1 (r=0.999 3), 3.048-91.44 ??g??mL^-1(r=0.999 4), 1.472-44.16 ??g??mL^-1(r=0.999 4), and 2.816-84.48 ??g??mL^-1 (r=0.999 2), respectively.The average recoveries (RSD) of the five compounds were 98.97%(1.03%), 99.90%(0.92%), 99.89% (1.75%), 99.55% (0.98%), and 99.76%(1.19%) (n=6), respectively. CONCLUSION The developed method is accurate and precise, which can be used for the quality control of different parts of Dracocephalum moldevica L.The RESULTS of content determination indicate that the five compounds exist in all the parts of Dracocephalum moldevica L., but the mass fractions are obviously different.