毛细管电泳法测定宁心宝胶囊中的核苷类成分

目的建立毛细管电泳法测定宁心宝胶囊中核苷类成分含量的方法。方法采用Agilent毛细管电泳仪,熔融石英毛细管56cm×75μm,有效长度50cm,自动压力(35mPa)进样3s,检测波长254nm,分离电压20kV,运行缓冲液为36mmol/L硼砂溶液-15mmol/L磷酸二氢钠溶液(pH9.2)。结果宁心宝胶囊中主要成分腺苷、鸟苷、尿苷、肌苷分别在10.1～252.5,10.3～258.0,10.3～257.6,10.8～270.0mg/L范围内有良好的线性关系,r分别为0.9998,0.9997,0.9996,0.9995。加样回收率分别为98.4%,102.5%,97.2%,101.3%。RSD分别为2.2%,1.8%,2.6%,2.0%。结论此法简便、快速,适用于宁心宝胶囊中核苷类成分的测定,为控制其质量提供了简便、易行的方法。

Determination of nucleosines in Ningxinbao Capsules by CE

Purpose To establish CE method for the assay of nucleosines in Ningxinbao Capsules.MethodsAgilent apparatus equipped with a UV detector and a untreated fused-quartz capillary(56 cm×75 μm,50 cm effective length)were used.Pressure injection was 35 mPa for 3 s,and the wavelength of detector was 254 nm.20 KV was applied for separation.The running buffer was 36 mmol/L Sodium borate-15 mmol/L Sodium dihydrogen phosphate(pH 9.2).ResultsThe linear ranges of adenosion,guanosion,uridine,inosion were in the concentration range of 10.1-252.5,10.3-258.0,10.3-257.6,and 10.8-270.0 mg/L,and r was 0.999 8,0.999 7,0.999 6,and 0.999 5 respectively.The average recoveries were 98.4%,102.5%,97.2% and 101.3%.RSD was 2.2%,1.8%,2.6%,and 2.0%,respectively.ConclusionThe method is simple,and rapid,and it is suitable for the assay of nucleosions in Ningxinbao Capsules.