Resuscitation and enumeration of Escherichia coli after freeze-drying |
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| Authors: | H. van Doorne Frederica S.C. van Voorst Vader |
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| Affiliation: | Department of Pharmaceutical Technology, Sub faculty of Pharmacy, State University of Leiden, P.O. Box 9502, 2300 RA LeidenThe Netherlands |
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| Abstract: | The suitability and precision of a recently suggested method for the resuscitation of Enterobacteriaceae is discussed. Experiments with freeze-dried cells of Escherichia coli ATCC 8739, showed that the recovery of the damaged cells was quantitative and that growth did not affect the result of the subsequent MPN count in Enterobacteriaceae Enrichment Broth. The method seems to be applicable for the examination of all kinds of products, and is possibly also suitable for the determination of other types of organisms. |
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