pSS患者唇腺细胞中Bcl-2的表达及其与细胞色素C的关系

目的：探讨原发性干燥综合征（pSS）患者唇腺细胞凋亡的发生机制。方法：收集临床患者，将其分为pSS组和非干燥综合征组。采用免疫组化的方法检测两组患者唇腺细胞Bcl一2蛋白的表达及含量；制备唇腺组织匀浆及提取线粒体，检测CytC的含量。使用SPSSl4．0软件对两组数据进行统计学分析。结果：pSS患者唇腺细胞中Bc1-2蛋白的表达和线粒体CytC的含量显著低于非干燥综合征组；pSS患者唇腺细胞胞浆CytC含量显著高于非干燥综合征组。结论：pSS的发病机制与Bcl-2蛋白及CytC诱导的细胞凋亡有关。

The expression of Bcl-2 and the relationship between the Bcl-2 and CytC in the labial gland of patients with primary Sjiigren＇s syndrome.

Objective： To explore the mechanism of apoptosis in the labial glands of patients with primary Sj6gren＇s syndrome （pSS）. Method： Collected the clinical patients, divided into pSS group and non-SS group.Immunohistochemistry was used to examine Bcl-2 protein in labial glands of two groups; The homogenate of labial glands was prepared, the mito- chondria was extracted, the CytC content was detected, SPSS14.0 software package was used for statistical analysis. Result： The expression of Bcl-2 and the mitochondria CytC with pSS were significantly lower than those in non-SS. The content of CytC in cytoplasm with pSS was significantly higher than that in non-SS. Conclusion： The pathogenesis of pSS is related to the apoptosis that induced by Bcl-2 protein and CytC.