精浆抗精子抗体阳性不育患者顶体酶、一氧化氮合酶及超氧化物歧化酶活力变化的研究

目的:研究精浆抗精子抗体(AsAb)阳性对精子顶体酶、精浆一氧化氮合酶(NOS)及超氧化物歧化酶(SOD)活力的影响。方法:精浆AsAb阳性不育者40例,对照组为40例正常生育男性。通过吸光度变化分别计算顶体酶活力(BAEE/ADH联合法)、NOS活力(氧化还原反应)、SOD活力(黄嘌呤氧化酶法)。结果:精浆As-Ab阳性组与正常生育组比较,精子顶体酶活力明显降低(P<0.01),NOS活力明显升高(P<0.01),精浆中SOD活力明显降低(P<0.01)。结论:精浆AsAb阳性引起不育可能与精子顶体酶、精浆中SOD及NOS活力改变有关。

Study of the Changes of Acrosomal Enzyme and Nitric Oxide Synthase, Superxide dismutase of Infertile Patients with Positive Antisperm Antibody in Seminal Plasma

Objective: To study the impacts of positive antisperm antibody(AsAb) in seminal plasma on acrosomal enzyme activity,nitric oxide synthase(NOS) and superxide dismutase(SOD) levels of spermatozoa. Methods: Swatch from 40 infertile patients with positive AsAb in seminal plasma as experimental group,and 40 fertile men as control group.Acrosomal enzyme activity was detected by the BAEE/ADH unitive method,NOS was detected by the redoxreaction assay,and SOD level was measured xanthine oxidase method. Results: Compared with control group,acrosomal enzyme activity of spermatozoa of experimental group was significantly decreased(P<(0.01)),NOS activity was apparently increased(P<(0.01)),and SOD level in seminal plasma was markedly decreased(P<(0.01)). Conclusion: It may be possible that the positive AsAb in seminal plasma beget infertility through the changes of acrosomal enzyme of spermatozoa,SOD and NOS activitise in seminal plasma.